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Mendeley readers
Attention Score in Context
| Title |
A Novel Selectable Islet 1 Positive Progenitor Cell Reprogrammed to Expandable and Functional Smooth Muscle Cells
|
|---|---|
| Published in |
Stem Cells, March 2016
|
| DOI | 10.1002/stem.2319 |
| Pubmed ID | |
| Authors |
Elizabeth C. Turner, Chien-Ling Huang, Neha Sawhney, Kalaimathi Govindarajan, Anthony J.P. Clover, Kenneth Martin, Tara C. Browne, Derek Whelan, Arun H.S. Kumar, John J. Mackrill, Shaohua Wang, Jeffrey Schmeckpeper, Alessia Stocca, William G. Pierce, Anne-Laure Leblond, Liquan Cai, Donnchadh M. O'Sullivan, Chirlei K. Buneker, Janet Choi, John MacSharry, Yasuhiro Ikeda, Stephen J. Russell, Noel M. Caplice |
| Abstract |
Disorders affecting smooth muscle structure/function may require technologies that can generate large scale, differentiated and contractile smooth muscle cells (SMC) suitable for cell therapy. To date no clonal precursor population that provides large numbers of differentiated SMC in culture has been identified in a rodent. Identification of such cells may also enhance insight into progenitor cell fate decisions and the relationship between smooth muscle precursors and disease states that implicate differentiated SMC. In this study we used classical clonal expansion techniques to identify novel self-renewing Islet 1 (Isl-1) positive primitive progenitor cells within rat bone marrow that exhibited canonical stem cell markers and preferential differentiation towards a smooth muscle-like fate. We subsequently used molecular tagging to select Isl-1 positive clonal populations from expanded and de-novo marrow cell populations. We refer to these previously undescribed cells as the primitive progenitor cell (PPC) given its stem cell marker profile, and robust self-renewal capacity. PPC could be directly converted into induced smooth muscle cells (iSMC) using single transcription factor (Kruppel-like factor 4) knockdown or transactivator (myocardin) overexpression in contrast to 3 control cells (HEK 293, endothelial cells and mesenchymal stem cells) where such induction was not possible. iSMC exhibited immuno- and cytoskeletal phenotype, calcium signalling profile and contractile responses similar to bona fide SMC. Passaged iSMC could be expanded to a scale sufficient for large scale tissue replacement. PPC and reprogrammed iSMC so derived may offer future opportunities to investigate molecular, structure/function and cell based replacement therapy approaches to diverse cardiovascular, respiratory, gastrointestinal and genitourinary diseases that have as their basis smooth muscle cell functional aberrancy or numerical loss. This article is protected by copyright. All rights reserved. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 15% |
| Other | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Student > Postgraduate | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Other | 3 | 15% |
| Unknown | 6 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 30% |
| Medicine and Dentistry | 3 | 15% |
| Environmental Science | 1 | 5% |
| Agricultural and Biological Sciences | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 6 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2016.
All research outputs
#15,355,821
of 22,842,950 outputs
Outputs from Stem Cells
#3,336
of 3,900 outputs
Outputs of similar age
#180,594
of 301,247 outputs
Outputs of similar age from Stem Cells
#70
of 87 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,900 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 301,247 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 87 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.