| Title |
MALDI-TOF MS in microbiological diagnostics—identification of microorganisms and beyond (mini review)
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, December 2011
|
| DOI | 10.1007/s00253-011-3783-4 |
| Pubmed ID | |
| Authors |
Andreas Wieser, Lukas Schneider, Jette Jung, Sören Schubert |
| Abstract |
Few developments in microbiological diagnostics have had such a rapid impact on species level identification of microorganisms as matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). Conventional differentiation methods rely on biochemical criteria and require additional pre-testing and lengthy incubation procedures. In comparison, MALDI-TOF MS can identify bacteria and yeast within minutes directly from colonies grown on culture plates. This radically new, methodically simple approach profoundly reduces the cost of consumables and time spent on diagnostics. The reliability and accuracy of the method have been demonstrated in numerous studies and different systems are already commercially available. Novel applications of the system besides microbial species level identification are also being explored. This includes identification of pathogens from positive blood cultures or directly from patient samples, such as urine. Currently, intriguing MALDI-TOF MS developments are being made regarding the phenotypic detection of certain antibiotic resistance mechanisms, e.g., β-lactamases and carbapenemases. This mini review provides an overview of the literature in the field and also includes our own data and experiences gathered from over 4 years of routine MALDI-TOF MS use in a university hospital's microbiological diagnostics facility. |
Mendeley readers
The data shown below were compiled from readership statistics for 631 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 3 | <1% |
| United States | 2 | <1% |
| United Kingdom | 2 | <1% |
| Germany | 1 | <1% |
| Netherlands | 1 | <1% |
| Finland | 1 | <1% |
| Canada | 1 | <1% |
| Colombia | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 615 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 120 | 19% |
| Researcher | 94 | 15% |
| Student > Ph. D. Student | 89 | 14% |
| Student > Master | 84 | 13% |
| Student > Doctoral Student | 35 | 6% |
| Other | 83 | 13% |
| Unknown | 126 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 138 | 22% |
| Biochemistry, Genetics and Molecular Biology | 87 | 14% |
| Medicine and Dentistry | 68 | 11% |
| Chemistry | 47 | 7% |
| Immunology and Microbiology | 42 | 7% |
| Other | 106 | 17% |
| Unknown | 143 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2020.
All research outputs
#5,089,278
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#1,221
of 8,034 outputs
Outputs of similar age
#42,573
of 250,231 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#19
of 91 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
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We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.