| Chapter title |
Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
|
|---|---|
| Chapter number | 8 |
| Book title |
Biotechnology of Plant Secondary Metabolism
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3393-8_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
| Authors |
Emmerstorfer-Augustin, Anita, Pichler, Harald, Anita Emmerstorfer-Augustin, Harald Pichler |
| Abstract |
Plant terpenoids are high-value compounds broadly applied as food additives or fragrances in perfumes and cosmetics. Their biotechnological production in yeast offers an attractive alternative to extraction from plants. Here, we provide two optimized protocols for the production of the plant terpenoid trans-nootkatol with recombinant S. cerevisiae by either (I) converting externally added (+)-valencene with resting cells or (II) cultivating engineered self-sufficient production strains. By synthesis of the hydrophobic compounds in self-sufficient production cells, phase transfer issues can be avoided and the highly volatile products can be enriched in and easily purified from n-dodecane, which is added to the cell broth as second phase. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 29% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Doctoral Student | 2 | 10% |
| Student > Master | 2 | 10% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 29% |
| Agricultural and Biological Sciences | 5 | 24% |
| Chemical Engineering | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Chemistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 7 | 33% |