Title |
The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes
|
---|---|
Published in |
Science, April 2008
|
DOI | 10.1126/science.1150021 |
Pubmed ID | |
Authors |
Maureen E. Hillenmeyer, Eula Fung, Jan Wildenhain, Sarah E. Pierce, Shawn Hoon, William Lee, Michael Proctor, Robert P. St.Onge, Mike Tyers, Daphne Koller, Russ B. Altman, Ronald W. Davis, Corey Nislow, Guri Giaever |
Abstract |
Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 17% |
United States | 1 | 17% |
Cameroon | 1 | 17% |
Germany | 1 | 17% |
Unknown | 2 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 67% |
Members of the public | 2 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 35 | 4% |
United Kingdom | 8 | 1% |
Germany | 5 | <1% |
France | 5 | <1% |
Switzerland | 3 | <1% |
Canada | 3 | <1% |
Mexico | 3 | <1% |
India | 3 | <1% |
Spain | 2 | <1% |
Other | 23 | 3% |
Unknown | 704 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 216 | 27% |
Researcher | 198 | 25% |
Student > Master | 66 | 8% |
Professor > Associate Professor | 53 | 7% |
Student > Bachelor | 51 | 6% |
Other | 136 | 17% |
Unknown | 74 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 447 | 56% |
Biochemistry, Genetics and Molecular Biology | 148 | 19% |
Computer Science | 24 | 3% |
Medicine and Dentistry | 20 | 3% |
Chemistry | 17 | 2% |
Other | 57 | 7% |
Unknown | 81 | 10% |