Title |
Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution
|
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Published in |
Nature Methods, January 2010
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DOI | 10.1038/nmeth.1414 |
Pubmed ID | |
Authors |
Hongcang Gu, Christoph Bock, Tarjei S Mikkelsen, Natalie Jäger, Zachary D Smith, Eleni Tomazou, Andreas Gnirke, Eric S Lander, Alexander Meissner |
Abstract |
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples. |
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Other | 6 | 1% |
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Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 143 | 34% |
Student > Ph. D. Student | 108 | 26% |
Professor > Associate Professor | 26 | 6% |
Student > Master | 25 | 6% |
Professor | 21 | 5% |
Other | 69 | 16% |
Unknown | 30 | 7% |
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Neuroscience | 6 | 1% |
Other | 35 | 8% |
Unknown | 46 | 11% |