| Title |
Genetic and physical maps around the sex-determining M-locus of the dioecious plant asparagus
|
|---|---|
| Published in |
Molecular Genetics and Genomics, July 2007
|
| DOI | 10.1007/s00438-007-0235-z |
| Pubmed ID | |
| Authors |
Alexa Telgmann-Rauber, Ari Jamsari, Michael S. Kinney, J. Chris Pires, Christian Jung |
| Abstract |
Asparagus officinalis L. is a dioecious plant. A region called the M-locus located on a pair of homomorphic sex chromosomes controls the sexual dimorphism in asparagus. The aim of this work was to clone the region determining sex in asparagus from its position in the genome. The structure of the region encompassing M should be investigated and compared to the sex-determining regions in other dioecious model species. To establish an improved basis for physical mapping, a high-resolution genetic map was enriched with AFLP markers closely linked to the target locus by carrying out a bulked segregant analysis. By screening a BAC library with AFLP- and STS-markers followed by chromosome walking, a physical map with eight contigs could be established. However, the gaps between the contigs could not be closed due to a plethora of repetitive elements. Surprisingly, two of the contigs on one side of the M-locus did not overlap although they have been established with two markers, which mapped in a distance as low as 0.25 cM flanking the sex locus. Thus, the clustering of the markers indicates a reduced recombination frequency within the M-region. On the opposite side of the M-locus, a contig was mapped in a distance of 0.38 cM. Four closely linked BAC clones were partially sequenced and 64 putative ORFs were identified. Interestingly, only 25% of the ORFs showed sequence similarity to known proteins and ESTs. In addition, an accumulation of repetitive sequences and a low gene density was revealed in the sex-determining region of asparagus. Molecular cytogenetic and sequence analysis of BACs flanking the M-locus indicate that the BACs contain highly repetitive sequences that localize to centromeric and pericentromeric locations on all asparagus chromosomes, which hindered the localization of the M-locus to the single pair of sex chromosomes. We speculate that dioecious Silene, papaya and Asparagus species may represent three stages in the evolution of XX, XY sex determination systems. Given that asparagus still rarely produces hermaphroditic flowers and has homomorphic sex chromosomes, this species may be an ideal system to further investigates early sex chromosome evolution and the origins of dioecy. |
Mendeley readers
The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | 2% |
| Italy | 1 | 2% |
| Germany | 1 | 2% |
| Costa Rica | 1 | 2% |
| Unknown | 60 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 23% |
| Researcher | 14 | 22% |
| Student > Master | 11 | 17% |
| Student > Bachelor | 5 | 8% |
| Professor | 4 | 6% |
| Other | 9 | 14% |
| Unknown | 6 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 42 | 66% |
| Biochemistry, Genetics and Molecular Biology | 10 | 16% |
| Social Sciences | 2 | 3% |
| Earth and Planetary Sciences | 1 | 2% |
| Environmental Science | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 8 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2024.
All research outputs
#2,655,919
of 25,374,917 outputs
Outputs from Molecular Genetics and Genomics
#34
of 3,319 outputs
Outputs of similar age
#5,911
of 78,482 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 8 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,319 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 78,482 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them