Title |
β-Catenin is a Nek2 substrate involved in centrosome separation
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Published in |
Genes & Development, December 2007
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DOI | 10.1101/gad.1596308 |
Pubmed ID | |
Authors |
Shirin Bahmanyar, Daniel D. Kaplan, Jennifer G. DeLuca, Thomas H. Giddings, Eileen T. O’Toole, Mark Winey, Edward D. Salmon, Patrick J. Casey, W. James Nelson, Angela I.M. Barth |
Abstract |
beta-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that beta-catenin is a component of interphase centrosomes and that stabilization of beta-catenin, mimicking mutations found in cancers, induces centrosome splitting. Centrosomes are held together by a dynamic linker regulated by Nek2 kinase and its substrates C-Nap1 (centrosomal Nek2-associated protein 1) and Rootletin. We show that beta-catenin binds to and is phosphorylated by Nek2, and is in a complex with Rootletin. In interphase, beta-catenin colocalizes with Rootletin between C-Nap1 puncta at the proximal end of centrioles, and this localization is dependent on C-Nap1 and Rootletin. In mitosis, when Nek2 activity increases, beta-catenin localizes to centrosomes at spindle poles independent of Rootletin. Increased Nek2 activity disrupts the interaction of Rootletin with centrosomes and results in binding of beta-catenin to Rootletin-independent sites on centrosomes, an event that is required for centrosome separation. These results identify beta-catenin as a component of the intercentrosomal linker and define a new function for beta-catenin as a key regulator of mitotic centrosome separation. |
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Germany | 2 | <1% |
Japan | 2 | <1% |
United Kingdom | 1 | <1% |
Portugal | 1 | <1% |
Canada | 1 | <1% |
Unknown | 197 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 51 | 24% |
Researcher | 46 | 22% |
Student > Master | 26 | 12% |
Professor > Associate Professor | 14 | 7% |
Student > Bachelor | 13 | 6% |
Other | 36 | 17% |
Unknown | 24 | 11% |
Readers by discipline | Count | As % |
---|---|---|
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Biochemistry, Genetics and Molecular Biology | 59 | 28% |
Medicine and Dentistry | 6 | 3% |
Physics and Astronomy | 3 | 1% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | <1% |
Other | 10 | 5% |
Unknown | 28 | 13% |