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A quantitative atlas of mitotic phosphorylation

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, August 2008
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Citations

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1383 Dimensions

Readers on

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1223 Mendeley
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11 CiteULike
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5 Connotea
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Title
A quantitative atlas of mitotic phosphorylation
Published in
Proceedings of the National Academy of Sciences of the United States of America, August 2008
DOI 10.1073/pnas.0805139105
Pubmed ID
Authors

Noah Dephoure, Chunshui Zhou, Judit Villén, Sean A. Beausoleil, Corey E. Bakalarski, Stephen J. Elledge, Steven P. Gygi

Abstract

The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this cycle. To gain further insight into how phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human cell line arrested in the G(1) and mitotic phases of the cell cycle. We report the identification of >14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed >1,000 proteins with increased phosphorylation in mitosis including many known cell cycle regulators. The majority of sites on regulated phosphopeptides lie in [S/T]P motifs, the minimum required sequence for CDKs, suggesting that many of the proteins may be CDK substrates. Analysis of non-proline site-containing phosphopeptides identified two unique motifs that suggest there are at least two undiscovered mitotic kinases.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1,223 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 21 2%
United Kingdom 8 <1%
Japan 6 <1%
France 5 <1%
Germany 4 <1%
Canada 4 <1%
Switzerland 3 <1%
Italy 2 <1%
Netherlands 2 <1%
Other 19 2%
Unknown 1149 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 374 31%
Researcher 252 21%
Student > Bachelor 133 11%
Student > Master 127 10%
Professor > Associate Professor 54 4%
Other 156 13%
Unknown 127 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 549 45%
Biochemistry, Genetics and Molecular Biology 373 30%
Medicine and Dentistry 49 4%
Chemistry 42 3%
Neuroscience 19 2%
Other 56 5%
Unknown 135 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 27. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2022.
All research outputs
#1,386,471
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#19,315
of 101,438 outputs
Outputs of similar age
#3,288
of 90,178 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#83
of 663 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 90,178 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 663 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.