Title |
B cell–intrinsic signaling through IL-21 receptor and STAT3 is required for establishing long-lived antibody responses in humans
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Published in |
The Journal of Experimental Medicine, January 2010
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DOI | 10.1084/jem.20091706 |
Pubmed ID | |
Authors |
Danielle T. Avery, Elissa K. Deenick, Cindy S., Santi Suryani, Nicholas Simpson, Gary Y. Chew, Tyani D. Chan, Umamainthan Palendira, Jacinta Bustamante, Stéphanie Boisson-Dupuis, Sharon Choo, Karl E. Bleasel, Jane Peake, Cecile King, Martyn A. French, Dan Engelhard, Sami Al-Hajjar, Saleh Al-Muhsen, Klaus Magdorf, Joachim Roesler, Peter D. Arkwright, Pravin Hissaria, D. Sean Riminton, Melanie Wong, Robert Brink, David A. Fulcher, Jean-Laurent Casanova, Matthew C. Cook, Stuart G. Tangye |
Abstract |
Engagement of cytokine receptors by specific ligands activate Janus kinase-signal transducer and activator of transcription (STAT) signaling pathways. The exact roles of STATs in human lymphocyte behavior remain incompletely defined. Interleukin (IL)-21 activates STAT1 and STAT3 and has emerged as a potent regulator of B cell differentiation. We have studied patients with inactivating mutations in STAT1 or STAT3 to dissect their contribution to B cell function in vivo and in response to IL-21 in vitro. STAT3 mutations dramatically reduced the number of functional, antigen (Ag)-specific memory B cells and abolished the ability of IL-21 to induce naive B cells to differentiate into plasma cells (PCs). This resulted from impaired activation of the molecular machinery required for PC generation. In contrast, STAT1 deficiency had no effect on memory B cell formation in vivo or IL-21-induced immunoglobulin secretion in vitro. Thus, STAT3 plays a critical role in generating effector B cells from naive precursors in humans. STAT3-activating cytokines such as IL-21 thus underpin Ag-specific humoral immune responses and provide a mechanism for the functional antibody deficit in STAT3-deficient patients. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 3 | 1% |
Netherlands | 2 | <1% |
Germany | 1 | <1% |
Brazil | 1 | <1% |
Australia | 1 | <1% |
United Kingdom | 1 | <1% |
Philippines | 1 | <1% |
Unknown | 260 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 69 | 26% |
Researcher | 56 | 21% |
Student > Bachelor | 19 | 7% |
Student > Postgraduate | 18 | 7% |
Student > Master | 18 | 7% |
Other | 52 | 19% |
Unknown | 38 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 73 | 27% |
Immunology and Microbiology | 64 | 24% |
Medicine and Dentistry | 41 | 15% |
Biochemistry, Genetics and Molecular Biology | 33 | 12% |
Engineering | 3 | 1% |
Other | 10 | 4% |
Unknown | 46 | 17% |