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Suppression of mRNAs Encoding Tegument Tetraspanins from Schistosoma mansoni Results in Impaired Tegument Turnover

Overview of attention for article published in PLoS Pathogens, April 2010
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Title
Suppression of mRNAs Encoding Tegument Tetraspanins from Schistosoma mansoni Results in Impaired Tegument Turnover
Published in
PLoS Pathogens, April 2010
DOI 10.1371/journal.ppat.1000840
Pubmed ID
Authors

Mai H. Tran, Tori C. Freitas, Leanne Cooper, Soraya Gaze, Michelle L. Gatton, Malcolm K. Jones, Erica Lovas, Edward J. Pearce, Alex Loukas

Abstract

Schistosomes express a family of integral membrane proteins, called tetraspanins (TSPs), in the outer surface membranes of the tegument. Two of these tetraspanins, Sm-TSP-1 and Sm-TSP-2, confer protection as vaccines in mice, and individuals who are naturally resistant to S. mansoni infection mount a strong IgG response to Sm-TSP-2. To determine their functions in the tegument of S. mansoni we used RNA interference to silence expression of Sm-tsp-1 and Sm-tsp-2 mRNAs. Soaking of parasites in Sm-tsp dsRNAs resulted in 61% (p = 0.009) and 74% (p = 0.009) reductions in Sm-tsp-1 and Sm-tsp-2 transcription levels, respectively, in adult worms, and 67%-75% (p = 0.011) and 69%-89% (p = 0.004) reductions in Sm-tsp-1 and Sm-tsp-2 transcription levels, respectively, in schistosomula compared to worms treated with irrelevant control (luciferase) dsRNA. Ultrastructural morphology of adult worms treated in vitro with Sm-tsp-2 dsRNA displayed a distinctly vacuolated and thinner tegument compared with controls. Schistosomula exposed in vitro to Sm-tsp-2 dsRNA had a significantly thinner and more vacuolated tegument, and morphology consistent with a failure of tegumentary invaginations to close. Injection of mice with schistosomula that had been electroporated with Sm-tsp-1 and Sm-tsp-2 dsRNAs resulted in 61% (p = 0.005) and 83% (p = 0.002) reductions in the numbers of parasites recovered from the mesenteries four weeks later when compared to dsRNA-treated controls. These results imply that tetraspanins play important structural roles impacting tegument development, maturation or stability.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Uruguay 1 1%
Australia 1 1%
United Kingdom 1 1%
Taiwan 1 1%
Japan 1 1%
Unknown 64 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 25%
Researcher 10 14%
Student > Postgraduate 7 10%
Student > Master 7 10%
Professor > Associate Professor 5 7%
Other 12 17%
Unknown 11 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 46%
Biochemistry, Genetics and Molecular Biology 9 13%
Immunology and Microbiology 8 12%
Medicine and Dentistry 5 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 4 6%
Unknown 10 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2010.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from PLoS Pathogens
#7,964
of 9,467 outputs
Outputs of similar age
#84,718
of 102,737 outputs
Outputs of similar age from PLoS Pathogens
#77
of 94 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 20.4. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 102,737 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.