| Title |
Identification and localization of the carboxysome peptide Csos3 and its corresponding gene in Thiobacillus neapolitanus
|
|---|---|
| Published in |
Archives of Microbiology, April 2000
|
| DOI | 10.1007/s002030000141 |
| Pubmed ID | |
| Authors |
Stefanie H. Baker, Donna S. Williams, Henry C. Aldrich, Alisa C. Gambrell, Jessup M. Shively |
| Abstract |
Four genes encoding carboxysome shell peptides (csoS1A, csoS1B, csoS1C, csoS2), the genes encoding the large and small subunits of RuBisCO (cbbL, cbbS), and three unidentified ORFs constitute an operon in Thiobacillus neapolitanus. An unidentified ORF 1.54 kb in size is predicted from sequence analysis to encode a protein with a molecular mass of approximately 57 kDa. When this ORF was expressed in Escherichia coli under the control of its endogenous ribosome-binding site, no peptide product was observed. In order to correlate this ORF with a carboxysome peptide, the ORF was overexpressed in E. coli by cloning it into pProExHTb, a prokaryotic expression vector containing an E. coli ribosome binding site. When antibodies raised against the recombinant protein were used to probe an immunoblot containing carboxysome peptides, a 60-kDa peptide was recognized. The peptide was subsequently named CsoS3. CsoS3 is a minor component of the carboxysome; a peptide of this size is commonly not observed or is very faint on Coomassie blue-stained SDS-polyacrylamide gels of purified carboxysomes. Immunogold labeling established CsoS3 to be a component of the carboxysome shell. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 2 | 7% |
| Czechia | 1 | 4% |
| United States | 1 | 4% |
| Unknown | 23 | 85% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 30% |
| Student > Ph. D. Student | 6 | 22% |
| Student > Postgraduate | 3 | 11% |
| Student > Master | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 37% |
| Biochemistry, Genetics and Molecular Biology | 8 | 30% |
| Immunology and Microbiology | 2 | 7% |
| Environmental Science | 1 | 4% |
| Earth and Planetary Sciences | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2019.
All research outputs
#4,836,164
of 25,374,647 outputs
Outputs from Archives of Microbiology
#237
of 3,119 outputs
Outputs of similar age
#6,029
of 40,972 outputs
Outputs of similar age from Archives of Microbiology
#3
of 17 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,119 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 40,972 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.