Title |
An assessment of histone-modification antibody quality
|
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Published in |
Nature Structural & Molecular Biology, December 2010
|
DOI | 10.1038/nsmb.1972 |
Pubmed ID | |
Authors |
Thea A Egelhofer, Aki Minoda, Sarit Klugman, Kyungjoon Lee, Paulina Kolasinska-Zwierz, Artyom A Alekseyenko, Ming-Sin Cheung, Daniel S Day, Sarah Gadel, Andrey A Gorchakov, Tingting Gu, Peter V Kharchenko, Samantha Kuan, Isabel Latorre, Daniela Linder-Basso, Ying Luu, Queminh Ngo, Marc Perry, Andreas Rechtsteiner, Nicole C Riddle, Yuri B Schwartz, Gregory A Shanower, Anne Vielle, Julie Ahringer, Sarah C R Elgin, Mitzi I Kuroda, Vincenzo Pirrotta, Bing Ren, Susan Strome, Peter J Park, Gary H Karpen, R David Hawkins, Jason D Lieb |
Abstract |
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 13 | 2% |
United Kingdom | 8 | 1% |
France | 4 | <1% |
Italy | 3 | <1% |
Spain | 3 | <1% |
Netherlands | 2 | <1% |
Germany | 2 | <1% |
Czechia | 2 | <1% |
Switzerland | 1 | <1% |
Other | 7 | 1% |
Unknown | 514 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 166 | 30% |
Researcher | 154 | 28% |
Student > Master | 43 | 8% |
Student > Bachelor | 31 | 6% |
Student > Doctoral Student | 28 | 5% |
Other | 91 | 16% |
Unknown | 46 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 296 | 53% |
Biochemistry, Genetics and Molecular Biology | 112 | 20% |
Medicine and Dentistry | 23 | 4% |
Chemistry | 22 | 4% |
Computer Science | 15 | 3% |
Other | 36 | 6% |
Unknown | 55 | 10% |