| Title |
Expression of fluconazole resistance-associated genes in biofilm from 23 clinical isolates of Candida albicans
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, January 2019
|
| DOI | 10.1007/s42770-018-0009-2 |
| Pubmed ID | |
| Authors |
Ce Shi, Jinyan Liu, Wenjing Li, Yue Zhao, Lingning Meng, Mingjie Xiang |
| Abstract |
This study aimed to establish the influence of biofilm from clinical isolates of Candida albicans on fluconazole resistance, focusing on efflux pumps and azole-targeted enzymes. Twenty-three C. albicans clinical isolates were collected from two hospitals in Shanghai, China. Antifungal susceptibility tests were performed on biofilm and planktonic cells. A crystal violet assay was used to monitor biofilm growth. Real-time RT-PCR was performed to quantify the expression of the transporter-related genes MDR1, CDR1, and CDR2 as well as ERG11, a gene encoding an enzyme targeted by antifungal drugs. Fluconazole resistance was shown to increase in biofilm in a time-dependent manner. No significant differences were observed between different strains of C. albicans. Genes encoding efflux pumps were overexpressed in early stages of biofilm formation and could also be induced by fluconazole. While ERG11 was not upregulated in biofilm, it was overexpressed upon the addition of fluconazole to biofilm and planktonic cells. Gene expression also appeared to be related to the original genotype of the strain. The upregulation of genes encoding efflux pumps demonstrates their role in the development of fluconazole resistance during the early stages of C. albicans biofilm formation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 14% |
| Student > Ph. D. Student | 3 | 10% |
| Student > Master | 3 | 10% |
| Student > Postgraduate | 2 | 7% |
| Professor > Associate Professor | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 10 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 6 | 21% |
| Agricultural and Biological Sciences | 6 | 21% |
| Biochemistry, Genetics and Molecular Biology | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 10 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2019.
All research outputs
#13,945,718
of 23,122,481 outputs
Outputs from Brazilian Journal of Microbiology
#376
of 1,135 outputs
Outputs of similar age
#222,923
of 437,003 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#2
of 17 outputs
Altmetric has tracked 23,122,481 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,135 research outputs from this source. They receive a mean Attention Score of 2.6. This one has gotten more attention than average, scoring higher than 66% of its peers.
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We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.