| Title |
Evaluation of growth and sporulation of a non-toxigenic strain of Clostridioides difficile (Z31) and its shelf viability
|
|---|---|
| Published in |
Brazilian Journal of Microbiology, December 2018
|
| DOI | 10.1007/s42770-018-0023-4 |
| Pubmed ID | |
| Authors |
Carlos Augusto Oliveira Júnior, Rodrigo Otávio Silveira Silva, Diogo Soares Gonçalves Cruz, Isadora Honorato Pires, Guilherme Guerra Alves, Francisco Carlos Faria Lobato |
| Abstract |
The oral administration of non-toxigenic strains of Clostridioides difficile (NTCD) is currently showing promising results for the prevention of Clostridioides difficile infection (CDI) in humans and animals, and is being considered as a possible commercial product to be used in the near future. The aim of this work was to evaluate five culture media for the growth and sporulation of one NTCD (Z31) and evaluate the viability of a lyophilized spore solution of NTCD Z31 stored at 4 °C or at 25 °C for 2 years. Reinforced clostridial medium (RCM) and brain heart infusion broth (BHI) provided the highest production of NTCD Z31 spores. In the first 6 months of the storage of the lyophilized solution, a reduction in spore count of approximately 0.3 Log10 CFU/mL was observed; however, no further significant reduction in spore count was observed up to 24 months. No difference in spore concentration was found between the two storage temperatures from 6 to 24 months of storage. The present work showed BHI and RCM to be the best choices for the growth and sporulation of NTCD Z31 and suggested that the spores of NTCD Z31 are stable for up to 2 years under both temperature conditions. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 29% |
| Other | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Master | 1 | 14% |
| Researcher | 1 | 14% |
| Other | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 4 | 57% |
| Agricultural and Biological Sciences | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2019.
All research outputs
#20,549,510
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Outputs from Brazilian Journal of Microbiology
#849
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Outputs of similar age
#370,197
of 435,349 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#13
of 17 outputs
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