| Title |
Adherence Inhibition of Cronobacter sakazakii to Intestinal Epithelial Cells by Prebiotic Oligosaccharides
|
|---|---|
| Published in |
Current Microbiology, February 2011
|
| DOI | 10.1007/s00284-011-9882-8 |
| Pubmed ID | |
| Authors |
Maria Quintero, Maria Maldonado, MariaElisa Perez-Munoz, Roberto Jimenez, Terry Fangman, John Rupnow, Anja Wittke, Michael Russell, Robert Hutkins |
| Abstract |
Cronobacter sakazakii is an opportunistic pathogen that has been implicated in meningitis, NEC, and sepsis in neonates. Colonization and subsequent infection and invasion of C. sakazakii require that the organism adheres to host cell surfaces. Agents that inhibit or block attachment of the pathogen to epithelial cells could be useful in reducing infections. The goal of this research was to assess the ability of prebiotic galactooligosaccharides (GOS) and polydextrose (PDX) to inhibit adherence of C. sakazakii 4603 to a HEp-2 human cell line. Adherence experiments were performed in the presence or absence of prebiotics using HEp-2 cells grown to confluency on glass coverslips. Prebiotics and bacteria were added and incubated for 3 h. Coverslips were washed, and adherence was determined by cultural and microscopic methods. When measured microscopically or by cultural methods, significant reductions in adherence (56 and 71%, respectively) of C. sakazakii were observed in the presence of GOS (16 mg/ml). Adherence inhibition also occurred (48%) when a GOS-PDX blend (8 mg/ml each) was tested, although PDX by itself had less effect. Similar results were also observed for Caco-2 cells and also for another strain of C. sakazakii (29004). These results suggest that GOS and PDX, alone and in combination, may have an anti-adhesive effect on C. sakazakii and directly inhibit the adherence to gastrointestinal epithelial cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Mexico | 1 | 1% |
| Argentina | 1 | 1% |
| Unknown | 84 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 23% |
| Researcher | 14 | 16% |
| Student > Master | 14 | 16% |
| Professor | 8 | 9% |
| Student > Bachelor | 5 | 6% |
| Other | 13 | 15% |
| Unknown | 13 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 44% |
| Chemistry | 7 | 8% |
| Immunology and Microbiology | 6 | 7% |
| Engineering | 4 | 5% |
| Medicine and Dentistry | 4 | 5% |
| Other | 8 | 9% |
| Unknown | 20 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2017.
All research outputs
#6,377,904
of 22,661,413 outputs
Outputs from Current Microbiology
#401
of 2,395 outputs
Outputs of similar age
#47,048
of 182,663 outputs
Outputs of similar age from Current Microbiology
#4
of 13 outputs
Altmetric has tracked 22,661,413 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 2,395 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 182,663 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.