| Title |
Chordoma and chondrosarcoma gene profile: implications for immunotherapy
|
|---|---|
| Published in |
Cancer Immunology, Immunotherapy, July 2008
|
| DOI | 10.1007/s00262-008-0557-7 |
| Pubmed ID | |
| Authors |
Joseph H. Schwab, Patrick J. Boland, Narasimhan P. Agaram, Nicholas D. Socci, Tianhua Guo, Gary C. O’Toole, Xinhui Wang, Elena Ostroumov, Christopher J. Hunter, Joel A. Block, Stephen Doty, Soldano Ferrone, John H. Healey, Cristina R. Antonescu |
| Abstract |
Chordoma and chondrosarcoma are malignant bone tumors characterized by the abundant production of extracellular matrix. The resistance of these tumors to conventional therapeutic modalities has prompted us to delineate the gene expression profile of these two tumor types, with the expectation to identify potential molecular therapeutic targets. Furthermore the transcriptional profile of chordomas and chrondrosarcomas was compared to a wide variety of sarcomas as well as to that of normal tissues of similar lineage, to determine whether they express unique gene signatures among other tumors of mesenchymal origin, and to identify changes associated with malignant transformation. A HG-U133A Affymetrix Chip platform was used to determine the gene expression signature in 6 chordoma and 14 chondrosarcoma lesions. Validation of selected genes was performed by qPCR and immunohistochemistry (IHC) on an extended subset of tumors. By unsupervised clustering, chordoma and chondrosarcoma tumors grouped together in a genomic cluster distinct from that of other sarcoma types. They shared overexpression of many extracellular matrix genes including aggrecan, type II & X collagen, fibronectin, matrillin 3, high molecular weight-melanoma associated antigen (HMW-MAA), matrix metalloproteinase MMP-9, and MMP-19. In contrast, T Brachyury and CD24 were selectively expressed in chordomas, as were Keratin 8,13,15,18 and 19. Chondrosarcomas are distinguished by high expression of type IX and XI collagen. Because of its potential usefulness as a target for immunotherapy, the expression of HMW-MAA was analyzed by IHC and was detected in 62% of chordomas and 48% of chondrosarcomas, respectively. Furthermore, western blotting analysis showed that HMW-MAA synthesized by chordoma cell lines has a structure similar to that of the antigen synthesized by melanoma cells. In conclusion, chordomas and chondrosarcomas share a similar gene expression profile of up-regulated extracellular matrix genes. HMW-MAA represents a potential useful target to apply immunotherapy to these tumors. |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 2% |
| Unknown | 60 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 21% |
| Student > Ph. D. Student | 11 | 18% |
| Professor > Associate Professor | 6 | 10% |
| Student > Master | 6 | 10% |
| Student > Postgraduate | 5 | 8% |
| Other | 12 | 20% |
| Unknown | 8 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 36% |
| Biochemistry, Genetics and Molecular Biology | 10 | 16% |
| Agricultural and Biological Sciences | 10 | 16% |
| Neuroscience | 5 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 11 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2021.
All research outputs
#2,954,420
of 22,899,952 outputs
Outputs from Cancer Immunology, Immunotherapy
#192
of 2,888 outputs
Outputs of similar age
#8,738
of 81,912 outputs
Outputs of similar age from Cancer Immunology, Immunotherapy
#1
of 22 outputs
Altmetric has tracked 22,899,952 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,888 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 81,912 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.