| Title |
Use of UTF1 Genetic Control Elements as iPSC Reporter
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, December 2011
|
| DOI | 10.1007/s12015-011-9342-7 |
| Pubmed ID | |
| Authors |
Amir Morshedi, Monireh Soroush Noghabi, Peter Dröge |
| Abstract |
The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and drug testing/development. An important aspect in the latter contexts is the ability to reliably monitor the pluripotent stem cell state, in particular with respect to human cell reprogramming using patient-specific somatic cells and high-throughput screens. Undifferentiated transcription factor 1 (UTF1) belongs to the core transcriptional network characterizing pluripotency. UTF1 is involved in ESC-specific chromatin organization, and its expression pattern during cell reprogramming and subsequent differentiation appears to be tightly connected with the pluripotent stem cell state. Here, we capitalized on these features and generated a reliable reporter system that was used to monitor induced pluripotent stem cell (iPSC) formation and subsequent differentiation. Our reporter cassette comprises less than 2.3 kb and remains functional during many cell passages after genomic integration. The fact that the human UTF1 genetic control elements work in a mouse background and the demonstrated functionality of the reporter in an epigenetic state further qualifies this system as a versatile new tool for iPSC research. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 4% |
| Australia | 1 | 4% |
| South Africa | 1 | 4% |
| Unknown | 20 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 17% |
| Researcher | 4 | 17% |
| Professor | 2 | 9% |
| Other | 2 | 9% |
| Professor > Associate Professor | 2 | 9% |
| Other | 4 | 17% |
| Unknown | 5 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 39% |
| Biochemistry, Genetics and Molecular Biology | 6 | 26% |
| Economics, Econometrics and Finance | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Materials Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 5 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2011.
All research outputs
#20,655,488
of 25,373,627 outputs
Outputs from Stem Cell Reviews and Reports
#820
of 1,035 outputs
Outputs of similar age
#203,598
of 249,527 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#9
of 9 outputs
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