↓ Skip to main content

Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia

Overview of attention for article published in Journal of Cell Biology, January 2012
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
92 Dimensions

Readers on

mendeley
70 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Ndfip1 regulates nuclear Pten import in vivo to promote neuronal survival following cerebral ischemia
Published in
Journal of Cell Biology, January 2012
DOI 10.1083/jcb.201105009
Pubmed ID
Authors

Jason Howitt, Jenny Lackovic, Ley-Hian Low, Adam Naguib, Alison Macintyre, Choo-Peng Goh, Jennifer K. Callaway, Vicki Hammond, Tim Thomas, Matthew Dixon, Ulrich Putz, John Silke, Perry Bartlett, Baoli Yang, Sharad Kumar, Lloyd C. Trotman, Seong-Seng Tan

Abstract

PTEN (phosphatase and tensin homologue deleted on chromosome TEN) is the major negative regulator of phosphatidylinositol 3-kinase signaling and has cell-specific functions including tumor suppression. Nuclear localization of PTEN is vital for tumor suppression; however, outside of cancer, the molecular and physiological events driving PTEN nuclear entry are unknown. In this paper, we demonstrate that cytoplasmic Pten was translocated into the nuclei of neurons after cerebral ischemia in mice. Critically, this transport event was dependent on a surge in the Nedd4 family-interacting protein 1 (Ndfip1), as neurons in Ndfip1-deficient mice failed to import Pten. Ndfip1 binds to Pten, resulting in enhanced ubiquitination by Nedd4 E3 ubiquitin ligases. In vitro, Ndfip1 overexpression increased the rate of Pten nuclear import detected by photobleaching experiments, whereas Ndfip1(-/-) fibroblasts showed negligible transport rates. In vivo, Ndfip1 mutant mice suffered larger infarct sizes associated with suppressed phosphorylated Akt activation. Our findings provide the first physiological example of when and why transient shuttling of nuclear Pten occurs and how this process is critical for neuron survival.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Unknown 69 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 22 31%
Researcher 13 19%
Student > Doctoral Student 5 7%
Student > Master 5 7%
Student > Bachelor 3 4%
Other 6 9%
Unknown 16 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 24%
Agricultural and Biological Sciences 17 24%
Neuroscience 10 14%
Medicine and Dentistry 3 4%
Engineering 2 3%
Other 7 10%
Unknown 14 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2012.
All research outputs
#17,286,645
of 25,374,917 outputs
Outputs from Journal of Cell Biology
#10,621
of 11,939 outputs
Outputs of similar age
#172,540
of 250,233 outputs
Outputs of similar age from Journal of Cell Biology
#47
of 72 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,939 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.6. This one is in the 4th percentile – i.e., 4% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,233 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.