| Title |
Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10
|
|---|---|
| Published in |
Archives of Microbiology, October 1990
|
| DOI | 10.1007/bf00245229 |
| Pubmed ID | |
| Authors |
Neil C. Bruce, Clare J. Wilmot, Keith N. Jordan, Anna E. Trebilcock, Lauren D. Gray Stephens, Christopher R. Lowe |
| Abstract |
A strain of Pseudomonas putida was isolated by selective enrichment with morphine that was capable of utilising morphine as a primary source of carbon and energy for growth. Experiments with whole cells showed that both morphine and codeine, but not thebaine, could be utilised. A novel NADP-dependent dehydrogenase, morphine dehydrogenase, was purified from crude cell extracts and was shown to be capable of oxidising morphine and codeine to morphinone and codeinone, respectively. This NADP-dependent morphine dehydrogenase was not observed in any other species of pseudomonads examined and was quite distinct from the beta-hydroxysteroid dehydrogenase found in Pseudomonas testosteroni, which had previously been shown to have activity against morphine. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 5% |
| Unknown | 20 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 33% |
| Student > Ph. D. Student | 5 | 24% |
| Student > Master | 3 | 14% |
| Other | 1 | 5% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 48% |
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Environmental Science | 2 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Unknown | 4 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2019.
All research outputs
#7,472,947
of 22,846,662 outputs
Outputs from Archives of Microbiology
#569
of 2,778 outputs
Outputs of similar age
#4,521
of 15,881 outputs
Outputs of similar age from Archives of Microbiology
#3
of 11 outputs
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