| Title |
Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons
|
|---|---|
| Published in |
Molecular Biology Reports, July 2009
|
| DOI | 10.1007/s11033-009-9615-2 |
| Pubmed ID | |
| Authors |
Hongxiu Wen, Xiqian Lan, Yuansong Zhang, Tianfu Zhao, Yujun Wang, Zenta Kajiura, Masao Nakagaki |
| Abstract |
Spider dragline silk is a unique fibrous protein with a combination of tensile strength and elasticity, but the isolation of large amounts of silk from spiders is not feasible. In this study, we generated germline-transgenic silkworms (Bombyx mori) that spun cocoons containing recombinant spider silk. A piggyBac-based transformation vector was constructed that carried spider dragline silk (MaSp1) cDNA driven by the sericin 1 promoter. Silkworm eggs were injected with the vector, producing transgenic silkworms displaying DsRed fluorescence in their eyes. Genotyping analysis confirmed the integration of the MaSp1 gene into the genome of the transgenic silkworms, and silk protein analysis revealed its expression and secretion in the cocoon. Compared with wild-type silk, the recombinant silk displayed a higher tensile strength and elasticity. The results indicate the potential for producing recombinant spider silk in transgenic B. mori. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| Japan | 1 | <1% |
| Russia | 1 | <1% |
| Unknown | 119 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 20% |
| Student > Bachelor | 24 | 19% |
| Researcher | 21 | 17% |
| Student > Master | 13 | 10% |
| Student > Doctoral Student | 8 | 6% |
| Other | 16 | 13% |
| Unknown | 17 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 50 | 40% |
| Biochemistry, Genetics and Molecular Biology | 26 | 21% |
| Chemistry | 8 | 6% |
| Engineering | 7 | 6% |
| Materials Science | 5 | 4% |
| Other | 8 | 6% |
| Unknown | 20 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 34. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2022.
All research outputs
#1,145,076
of 24,953,268 outputs
Outputs from Molecular Biology Reports
#16
of 3,206 outputs
Outputs of similar age
#3,196
of 117,453 outputs
Outputs of similar age from Molecular Biology Reports
#1
of 24 outputs
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So far Altmetric has tracked 3,206 research outputs from this source. They receive a mean Attention Score of 2.3. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.