| Title |
Neurite-Enriched MicroRNA-218 Stimulates Translation of the GluA2 Subunit and Increases Excitatory Synaptic Strength
|
|---|---|
| Published in |
Molecular Neurobiology, January 2019
|
| DOI | 10.1007/s12035-019-1492-7 |
| Pubmed ID | |
| Authors |
Anna Rocchi, Daniela Moretti, Gabriele Lignani, Elisabetta Colombo, Joachim Scholz-Starke, Pietro Baldelli, Tatiana Tkatch, Fabio Benfenati |
| Abstract |
Local control of protein translation is a fundamental process for the regulation of synaptic plasticity. It has been demonstrated that the local protein synthesis occurring in axons and dendrites can be shaped by numerous mechanisms, including miRNA-mediated regulation. However, several aspects underlying this regulatory process have not been elucidated yet. Here, we analyze the differential miRNA profile in cell bodies and neurites of primary hippocampal neurons and find an enrichment of the precursor and mature forms of miR-218 in the neuritic projections. We show that miR-218 abundance is regulated during hippocampal development and by chronic silencing or activation of neuronal network. Overexpression and knockdown of miR-218 demonstrated that miR-218 targets the mRNA encoding the GluA2 subunit of AMPA receptors and modulates its expression. At the functional level, miR-218 overexpression increases glutamatergic synaptic transmission at both single neuron and network levels. Our data demonstrate that miR-218 may play a key role in the regulation of AMPA-mediated excitatory transmission and in the homeostatic regulation of synaptic plasticity. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 75% |
| Members of the public | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 26% |
| Student > Ph. D. Student | 7 | 18% |
| Student > Doctoral Student | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Lecturer | 1 | 3% |
| Other | 5 | 13% |
| Unknown | 10 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 14 | 37% |
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Agricultural and Biological Sciences | 3 | 8% |
| Psychology | 2 | 5% |
| Unspecified | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 10 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2019.
All research outputs
#12,822,979
of 23,125,690 outputs
Outputs from Molecular Neurobiology
#1,602
of 3,500 outputs
Outputs of similar age
#195,032
of 437,851 outputs
Outputs of similar age from Molecular Neurobiology
#37
of 69 outputs
Altmetric has tracked 23,125,690 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 69 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.