| Title |
Haemolysis affects insulin but not C-peptide immunoassay
|
|---|---|
| Published in |
Diabetologia, June 1987
|
| DOI | 10.1007/bf00292540 |
| Pubmed ID | |
| Authors |
S. O'Rahilly, M. A. Burnett, R. F. Smith, J. H. Darley, R. C. Turner |
| Abstract |
Venous blood was taken at the end of a glucose infusion test in 19 individuals and divided into four aliquots, 3 of which were variably haemolysed by repeated passage through a 23-gauge needle to simulate traumatic venepuncture. Plasma insulin (measured by both a charcoal separation and a double-antibody method), C-peptide, and haemoglobin were measured in each aliquot, and haemolysis was also assessed visibly. A significant loss of immuno-assayable plasma insulin was found in samples with only a trace of visible haemolysis, with up to 90% lost in severely haemolysed samples. Plasma C-peptide was unaffected by haemolysis. This represents an additional advantage for the use of plasma C-peptide in assessing insulin secretion. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 20% |
| Researcher | 3 | 12% |
| Other | 2 | 8% |
| Student > Postgraduate | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 8 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 10 | 40% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Agricultural and Biological Sciences | 2 | 8% |
| Computer Science | 1 | 4% |
| Unknown | 10 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 1996.
All research outputs
#7,472,947
of 22,846,662 outputs
Outputs from Diabetologia
#2,834
of 5,035 outputs
Outputs of similar age
#3,404
of 12,140 outputs
Outputs of similar age from Diabetologia
#3
of 4 outputs
Altmetric has tracked 22,846,662 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,035 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.7. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one.