↓ Skip to main content

Mitochondrial DNA Damage in Iron Overload*

Overview of attention for article published in Journal of Biological Chemistry, December 2008
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

twitter
5 X users
patent
1 patent
facebook
2 Facebook pages

Citations

dimensions_citation
98 Dimensions

Readers on

mendeley
74 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Mitochondrial DNA Damage in Iron Overload*
Published in
Journal of Biological Chemistry, December 2008
DOI 10.1074/jbc.m806235200
Pubmed ID
Authors

Xueshan Gao, Jian Li Campian, Mingwei Qian, Xiao-Feng Sun, John W. Eaton

Abstract

Chronic iron overload has slow and insidious effects on heart, liver, and other organs. Because iron-driven oxidation of most biologic materials (such as lipids and proteins) is readily repaired, this slow progression of organ damage implies some kind of biological "memory." We hypothesized that cumulative iron-catalyzed oxidant damage to mtDNA might occur in iron overload, perhaps explaining the often lethal cardiac dysfunction. Real time PCR was used to examine the "intactness" of mttDNA in cultured H9c2 rat cardiac myocytes. After 3-5 days exposure to high iron, these cells exhibited damage to mtDNA reflected by diminished amounts of near full-length 15.9-kb PCR product with no change in the amounts of a 16.1-kb product from a nuclear gene. With the loss of intact mtDNA, cellular respiration declined and mRNAs for three electron transport chain subunits and 16 S rRNA encoded by mtDNA decreased, whereas no decrements were found in four subunits encoded by nuclear DNA. To examine the importance of the interactions of iron with metabolically generated reactive oxygen species, we compared the toxic effects of iron in wild-type and rho(o) cells. In wild-type cells, elevated iron caused increased production of reactive oxygen species, cytostasis, and cell death, whereas the rho(o) cells were unaffected. We conclude that long-term damage to cells and organs in iron-overload disorders involves interactions between iron and mitochondrial reactive oxygen species resulting in cumulative damage to mtDNA, impaired synthesis of respiratory chain subunits, and respiratory dysfunction.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 1%
Unknown 73 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 23 31%
Student > Bachelor 12 16%
Student > Ph. D. Student 10 14%
Student > Master 8 11%
Other 6 8%
Other 8 11%
Unknown 7 9%
Readers by discipline Count As %
Medicine and Dentistry 21 28%
Agricultural and Biological Sciences 14 19%
Biochemistry, Genetics and Molecular Biology 14 19%
Chemistry 6 8%
Immunology and Microbiology 2 3%
Other 6 8%
Unknown 11 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2023.
All research outputs
#4,835,465
of 25,371,288 outputs
Outputs from Journal of Biological Chemistry
#12,344
of 85,238 outputs
Outputs of similar age
#25,283
of 181,060 outputs
Outputs of similar age from Journal of Biological Chemistry
#65
of 397 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 85,238 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 181,060 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 397 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.