| Title |
Tumor necrosis-initiated complement activation stimulates proliferation of medulloblastoma cells
|
|---|---|
| Published in |
Inflammation Research, January 2015
|
| DOI | 10.1007/s00011-015-0796-y |
| Pubmed ID | |
| Authors |
Adrian J. Maurer, Phillip A. Bonney, Lucas C. Toho, Chad A. Glenn, Shweta Agarwal, James D. Battiste, Kar-Ming Fung, Michael E. Sughrue |
| Abstract |
We sought to determine the effect of necrosis-induced activation of the complement protein C3 in medulloblastoma. Twelve medulloblastoma surgical specimens were evaluated for complement activation using immunohistochemistry, with H&E stains performed on adjacent tissue sections to determine the relationship of complement activation to necrotic tissue. Flow cytometry and Western blot were performed on three established medulloblastoma lines and one surgically-procured cell culture to determine expression of C3a receptor (C3aR) in medulloblastoma. In vitro proliferation of siRNA C3aR knockdown cells was compared to that of control siRNA cells with cell line Daoy. Three surgical specimens were found to have necrosis on H&E sections. In each case, iC3b staining was identified on adjacent sections, limited to the necrotic region. In no case did necrosis occur without iC3b staining on adjacent sections. C3aR protein was demonstrated on both the three established cell lines and on the surgical culture. Proliferation assays of Daoy cells with siRNA knockdown vs. control siRNA revealed significantly reduced proliferation at 72 h (p = 0.001). Necrosis is associated with complement activation in medulloblastoma. Medulloblastoma cells express C3aR, and siRNA-mediated knockdown of C3aR inhibits proliferation of these cells in vitro. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 2 | 17% |
| Student > Bachelor | 2 | 17% |
| Student > Master | 2 | 17% |
| Other | 1 | 8% |
| Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 3 | 25% |
| Agricultural and Biological Sciences | 2 | 17% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Unknown | 5 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2016.
All research outputs
#20,306,690
of 22,846,662 outputs
Outputs from Inflammation Research
#786
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Outputs of similar age
#295,875
of 352,084 outputs
Outputs of similar age from Inflammation Research
#12
of 14 outputs
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