Title |
Cross-Sectional Characterization of Albumin Glycation State in Cerebrospinal Fluid and Plasma from Alzheimer’s Disease Patients
|
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Published in |
The Journal of Prevention of Alzheimer's Disease, December 2018
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DOI | 10.14283/jpad.2018.48 |
Pubmed ID | |
Authors |
Montserrat Costa, A. Mestre, R. Horrillo, A. M. Ortiz, A. Pérez, A. Ruiz, M. Boada, S. Grancha |
Abstract |
We determined albumin post-translational modifications (PTMs) by mass spectrometry (MS) in plasma and cerebrospinal fluid (CSF) from 31 Alzheimer's disease (AD) patients (with 27 samples of paired plasma-CSF from the same patients). Results were cross-sectionally compared with healthy controls. For percentage of relative intensity of glycated isoforms, plasma albumin was globally more glycated in AD patients than in healthy controls (P<0.01). MS results in plasma were confirmed by a quantitative enzymatic assay (Lucica GA-L) for albumin early-glycation detection. In CSF there were no global glycation differences detected by MS, although a different pattern of glycated isoforms was observed. Oxidized+glycated and cysteinylated+glycated isoforms were increased in both plasma and CSF of AD patients in comparison with healthy controls (P<0.001). Furthermore, AD patients showed higher glycation in plasma than in CSF (P<0.01). Our data support the role of glycation and oxidative stress in AD. |
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France | 3 | 38% |
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Unknown | 3 | 38% |
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Science communicators (journalists, bloggers, editors) | 2 | 25% |
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Mendeley readers
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Other | 1 | 6% |
Lecturer | 1 | 6% |
Lecturer > Senior Lecturer | 1 | 6% |
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Other | 3 | 18% |
Unknown | 8 | 47% |
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Other | 3 | 18% |
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