| Title |
Expression and Production of Therapeutic Recombinant Human Platelet-Derived Growth Factor-BB in Pleurotus eryngii
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, May 2011
|
| DOI | 10.1007/s12010-011-9279-y |
| Pubmed ID | |
| Authors |
Jun-Hui Choi, Seung Kim, Kumar Sapkota, Se-Eun Park, Sung-Jun Kim |
| Abstract |
Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) is widely used in many therapeutic applications. Until now, there has been no report on rhPDGF-BB expressed in fungi. In this study, we tested whether Pleurotus eryngii could support the expression of human therapeutic rhPDGF-BB protein. A binary vector pCAMBIA1304 containing the hPDGF-BB gene was constructed and introduced into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformation of hPDGF-BB gene was confirmed by Southern blot and PCR, whereas the expression was confirmed by Western blot analysis. The recombinant hPDGF-BB reached a maximum expression level of 1.98% of total soluble protein in transgenic mycelia and was in dimeric form. A bioassay revealed that hPDGF-BB expressed in P. eryngii increased proliferation of NIH-3T3 cells similarly to standard material. These results suggest that P. eryngii can be a robust system for the production of human therapeutic proteins including the hPDGF-BB. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 5% |
| Unknown | 19 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 30% |
| Student > Ph. D. Student | 4 | 20% |
| Professor | 3 | 15% |
| Student > Bachelor | 2 | 10% |
| Student > Master | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 25% |
| Biochemistry, Genetics and Molecular Biology | 3 | 15% |
| Computer Science | 2 | 10% |
| Social Sciences | 2 | 10% |
| Immunology and Microbiology | 2 | 10% |
| Other | 3 | 15% |
| Unknown | 3 | 15% |