| Title |
Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae
|
|---|---|
| Published in |
Current Genetics, October 1995
|
| DOI | 10.1007/bf00310817 |
| Pubmed ID | |
| Authors |
Johan M. Crous, Isak S. Pretorius, Willem H. van Zyl |
| Abstract |
First-strand cDNA was prepared from mRNA isolated from Aspergillus kawachii IFO4308 and the beta-xylanase gene (xynC) amplified by using the polymerase chain reaction (PCR) technique. This gene was inserted between the yeast phosphoglycerate kinase (PGK1) gene promoter (PGK1p) and terminator (PGK1T) sequences. The PGK1p-xynC-PGK1T construct (designated XYN3) was cloned into a multicopy episomal plasmid and the XYN3 gene was expressed in Saccharomyces cerevisiae. Functional beta-xylanase (Xyn3) was produced and secreted by the recombinant yeast. Xyn3 was stable between 30 and 50 degrees C, and the optimum temperature and pH were shown to be at 60 degrees C and lower than pH 3, respectively. An autoselective furl::LEU2 XYN3 recombinant strain was developed that allowed beta-xylanase production at a level of 300 nkat/ml in a non-selective complex medium. |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 3% |
| Portugal | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 31 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 21% |
| Researcher | 7 | 21% |
| Professor | 4 | 12% |
| Student > Postgraduate | 3 | 9% |
| Student > Master | 3 | 9% |
| Other | 7 | 21% |
| Unknown | 3 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 62% |
| Engineering | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Social Sciences | 1 | 3% |
| Chemistry | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 5 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2022.
All research outputs
#4,851,051
of 23,377,816 outputs
Outputs from Current Genetics
#121
of 1,208 outputs
Outputs of similar age
#3,047
of 24,552 outputs
Outputs of similar age from Current Genetics
#1
of 7 outputs
Altmetric has tracked 23,377,816 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,208 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 24,552 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them