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Attention Score in Context
| Title |
Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1
|
|---|---|
| Published in |
Microbiology, May 2003
|
| DOI | 10.1099/mic.0.25967-0 |
| Pubmed ID | |
| Authors |
Amanda S Nouwens, Scott A Beatson, Cynthia B Whitchurch, Bradley J Walsh, Herbert P Schweizer, John S Mattick, Stuart J Cordwell |
| Abstract |
The las and rhl quorum sensing (QS) systems regulate the expression of several genes in response to cell density changes in Pseudomonas aeruginosa. Many of these genes encode surface-associated or secreted virulence factors. Proteins from stationary phase culture supernatants were collected from wild-type and P. aeruginosa PAO1 mutants deficient in one or more of the lasRI, rhlRI and vfr genes and analysed using two-dimensional gel electrophoresis. All mutants released significantly lower amounts of protein than the wild-type. Protein spot patterns from each strain were compared using image analysis and visible spot differences were identified using mass spectrometry. Several previously unknown QS-regulated proteins were characterized, including an aminopeptidase (PA2939), an endoproteinase (PrpL) and a unique 'hypothetical' protein (PA0572), which could not be detected in the culture supernatants of Deltalas mutants, although they were unaffected in Deltarhl mutants. Chitin-binding protein (CbpD) and a hypothetical protein (PA4944) with similarity to host factor I (HF-I) could not be detected when any of the lasRI or rhlRI genes were disrupted. Fourteen proteins were present at significantly greater levels in the culture supernatants of QS mutants, suggesting that QS may also negatively control the expression of some genes. Increased levels of two-partner secretion exoproteins (PA0041 and PA4625) were observed and may be linked to increased stability of their cognate transporters in a QS-defective background. Known QS-regulated extracellular proteins, including elastase (lasB), LasA protease (lasA) and alkaline metalloproteinase (aprA) were also detected. |
Mendeley readers
The data shown below were compiled from readership statistics for 150 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 3% |
| Germany | 1 | <1% |
| Chile | 1 | <1% |
| Brazil | 1 | <1% |
| France | 1 | <1% |
| Belgium | 1 | <1% |
| United Kingdom | 1 | <1% |
| Unknown | 139 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 38 | 25% |
| Researcher | 28 | 19% |
| Student > Master | 17 | 11% |
| Professor > Associate Professor | 15 | 10% |
| Student > Doctoral Student | 14 | 9% |
| Other | 24 | 16% |
| Unknown | 14 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 67 | 45% |
| Biochemistry, Genetics and Molecular Biology | 22 | 15% |
| Immunology and Microbiology | 19 | 13% |
| Medicine and Dentistry | 6 | 4% |
| Chemistry | 5 | 3% |
| Other | 8 | 5% |
| Unknown | 23 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 June 2018.
All research outputs
#6,869,233
of 24,003,070 outputs
Outputs from Microbiology
#1,432
of 3,837 outputs
Outputs of similar age
#15,911
of 52,055 outputs
Outputs of similar age from Microbiology
#8
of 21 outputs
Altmetric has tracked 24,003,070 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 3,837 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 52,055 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.