| Title |
Identification of proteins derived from Listeria monocytogenes inducing human dendritic cell maturation
|
|---|---|
| Published in |
Tumor Biology, February 2016
|
| DOI | 10.1007/s13277-016-4933-1 |
| Pubmed ID | |
| Authors |
Reza Mirzaei, Azad Saei, Fatemeh Torkashvand, Bahareh Azarian, Ahmad Jalili, Farshid Noorbakhsh, Behrouz Vaziri, Jamshid Hadjati |
| Abstract |
Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that can promote antitumor immunity when pulsed with tumor antigens and then matured by stimulatory agents. Despite apparent progress in DC-based cancer immunotherapy, some discrepancies were reported in generating potent DCs. Listeria monocytogenes as an intracellular microorganism is able to effectively activate DCs through engaging pattern-recognition receptors (PRRs). This study aimed to find the most potent components derived from L. monocytogenes inducing DC maturation. The preliminary results demonstrated that the ability of protein components is higher than DNA components to promote DC maturation and activation. Protein lysate fractionation demonstrated that fraction 2 HIC (obtained by hydrophobic interaction chromatography) was able to efficiently mature DCs. F2HIC-matured DCs are able to induce allogeneic CD8(+) T cells proliferation better than LPS-matured DCs and induce IFN-γ producing CD8(+) T cells. Mass spectrometry results showed that F2HIC contains 109 proteins. Based on the bioinformatics analysis for these 109 proteins, elongation factor Tu (EF-Tu) could be considered as a PRR ligand for stimulating DC maturation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 3 | 25% |
| Professor > Associate Professor | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Lecturer | 1 | 8% |
| Other | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 5 | 42% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Neuroscience | 2 | 17% |
| Computer Science | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2016.
All research outputs
#16,122,040
of 23,923,788 outputs
Outputs from Tumor Biology
#1,084
of 2,624 outputs
Outputs of similar age
#179,929
of 301,105 outputs
Outputs of similar age from Tumor Biology
#40
of 163 outputs
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So far Altmetric has tracked 2,624 research outputs from this source. They receive a mean Attention Score of 2.4. This one has gotten more attention than average, scoring higher than 51% of its peers.
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We're also able to compare this research output to 163 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.