| Title |
Integrated molecular, physiological and in silico characterization of two Halomonas isolates from industrial brine
|
|---|---|
| Published in |
Extremophiles, February 2016
|
| DOI | 10.1007/s00792-015-0806-6 |
| Pubmed ID | |
| Authors |
Ross P. Carlson, Olusegun Oshota, Matt Shipman, Justin A. Caserta, Ping Hu, Charles W. Saunders, Jun Xu, Zackary J. Jay, Nancy Reeder, Abigail Richards, Charles Pettigrew, Brent M. Peyton |
| Abstract |
Two haloalkaliphilic bacteria isolated from industrial brine solutions were characterized via molecular, physiological, and in silico metabolic pathway analyses. Genomes from the organisms, designated Halomonas BC1 and BC2, were sequenced; 16S ribosomal subunit-based phylogenetic analysis revealed a high level of similarity to each other and to Halomonas meridiana. Both strains were moderate halophiles with near optimal specific growth rates (≥60 % μ max) observed over <0.1-5 % (w/v) NaCl and pH ranging from 7.4 to 10.2. Isolate BC1 was further characterized by measuring uptake or synthesis of compatible solutes under different growth conditions; in complex medium, uptake and accumulation of external glycine betaine was observed while ectoine was synthesized de novo in salts medium. Transcriptome analysis of isolate BC1 grown on glucose or citrate medium measured differences in glycolysis- and gluconeogenesis-based metabolisms, respectively. The annotated BC1 genome was used to build an in silico, genome-scale stoichiometric metabolic model to study catabolic energy strategies and compatible solute synthesis under gradients of oxygen and nutrient availability. The theoretical analysis identified energy metabolism challenges associated with acclimation to high salinity and high pH. The study documents central metabolism data for the industrially and scientifically important haloalkaliphile genus Halomonas. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 32% |
| Student > Master | 5 | 20% |
| Other | 2 | 8% |
| Researcher | 2 | 8% |
| Student > Doctoral Student | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 5 | 20% |
| Agricultural and Biological Sciences | 4 | 16% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Chemical Engineering | 2 | 8% |
| Immunology and Microbiology | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 7 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 May 2016.
All research outputs
#15,359,595
of 22,849,304 outputs
Outputs from Extremophiles
#543
of 799 outputs
Outputs of similar age
#176,591
of 298,010 outputs
Outputs of similar age from Extremophiles
#8
of 18 outputs
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