| Title |
Microarray sampling-platform fabrication using bubble-jet technology for a biochip system
|
|---|---|
| Published in |
Fresenius' Journal of Analytical Chemistry, February 2014
|
| DOI | 10.1007/s002160100962 |
| Pubmed ID | |
| Authors |
Leonardo R. Allain, Minoo Askari, David L. Stokes, Tuan Vo-Dinh |
| Abstract |
The fabrication of microarrays containing PCR-amplified genomic DNA extracts from mice tumors on a Zetaprobe membrane using a modified thermal ink-jet printer is described. A simple and cost-effective procedure for the fabrication of microarrays containing biological samples using a modified bubble-jet printing system is presented. Because of their mass-produced design, ink-jet printers are a much cheaper alternative to conventional spotting techniques. The usefulness of the biochip microarray platform is illustrated by the detection of human fragile histidine triad (FHIT), a tumor suppressor gene. Subcutaneous carcinomas were induced with MKN/FHIT and MKN/E4 cell lines in immunodeficient mice. Several weeks into their development, the tumors from both groups of mice were removed and subjected to DNA extraction by lysis of tissue samples. The extracted DNA samples were amplified by PCR (30 cycles) using the primers corresponding to nucleotides 2 to 18 of the FHIT sequence. The resulting solution was transferred to the individual reservoirs of a three-color cartridge from a conventional thermal ink-jet printer (HP 694C), and arrays were printed on to a Zetaprobe membrane. After spotting, these membranes were used in a hybridization assay, using fluorescent probes, and detected with a biochip. |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Japan | 1 | 3% |
| Unknown | 38 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 43% |
| Researcher | 7 | 18% |
| Professor | 3 | 8% |
| Other | 2 | 5% |
| Student > Bachelor | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 12 | 30% |
| Chemistry | 8 | 20% |
| Agricultural and Biological Sciences | 5 | 13% |
| Physics and Astronomy | 3 | 8% |
| Computer Science | 1 | 3% |
| Other | 6 | 15% |
| Unknown | 5 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2014.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Fresenius' Journal of Analytical Chemistry
#38
of 134 outputs
Outputs of similar age
#100,402
of 329,374 outputs
Outputs of similar age from Fresenius' Journal of Analytical Chemistry
#6
of 14 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 134 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 3rd percentile – i.e., 3% of its peers scored the same or lower than it.
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We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.