| Title |
A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins
|
|---|---|
| Published in |
Journal of Biomolecular NMR, May 2001
|
| DOI | 10.1023/a:1011258906244 |
| Pubmed ID | |
| Authors |
Pei Zhou, Alexey A. Lugovskoy, Gerhard Wagner |
| Abstract |
Protein-fusion constructs have been used with great success for enhancing expression of soluble recombinant protein and as tags for affinity purification. Unfortunately the most popular tags, such as GST and MBP, are large, which hinders direct NMR studies of the fusion proteins. Cleavage of the fusion proteins often re-introduces problems with solubility and stability. Here we describe the use of N-terminally fused protein G (B1 domain) as a non-cleavable solubility-enhancement tag (SET) for structure determination of a dimeric protein complex. The SET enhances the solubility and stability of the fusion product dramatically while not interacting directly with the protein of interest. This approach can be used for structural characterization of poorly behaving protein systems, and would be especially useful for structural genomics studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 125 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 4% |
| United Kingdom | 1 | <1% |
| Germany | 1 | <1% |
| Slovenia | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 116 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 41 | 33% |
| Student > Ph. D. Student | 27 | 22% |
| Student > Master | 10 | 8% |
| Student > Bachelor | 10 | 8% |
| Professor > Associate Professor | 7 | 6% |
| Other | 19 | 15% |
| Unknown | 11 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 56 | 45% |
| Biochemistry, Genetics and Molecular Biology | 31 | 25% |
| Chemistry | 12 | 10% |
| Engineering | 3 | 2% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
| Other | 7 | 6% |
| Unknown | 14 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 August 2014.
All research outputs
#5,446,210
of 25,371,288 outputs
Outputs from Journal of Biomolecular NMR
#66
of 561 outputs
Outputs of similar age
#7,096
of 42,351 outputs
Outputs of similar age from Journal of Biomolecular NMR
#1
of 5 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 561 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 84% of its peers.
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We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them