| Title |
Reliable Determinations of Protein–Ligand Interactions by Direct ESI-MS Measurements. Are We There Yet?
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, January 2012
|
| DOI | 10.1007/s13361-011-0311-9 |
| Pubmed ID | |
| Authors |
Elena N. Kitova, Amr El-Hawiet, Paul D. Schnier, John S. Klassen |
| Abstract |
The association-dissociation of noncovalent interactions between protein and ligands, such as other proteins, carbohydrates, lipids, DNA, or small molecules, are critical events in many biological processes. The discovery and characterization of these interactions is essential to a complete understanding of biochemical reactions and pathways and to the design of novel therapeutic agents that may be used to treat a variety of diseases and infections. Over the last 20 y, electrospray ionization mass spectrometry (ESI-MS) has emerged as a versatile tool for the identification and quantification of protein-ligand interactions in vitro. Here, we describe the implementation of the direct ESI-MS assay for the determination of protein-ligand binding stoichiometry and affinity. Additionally, we outline common sources of error encountered with these measurements and various strategies to overcome them. Finally, we comment on some of the outstanding challenges associated with the implementation of the assay and highlight new areas where direct ESI-MS measurements are expected to make significant contributions in the future. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 136 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| Chile | 1 | <1% |
| United Kingdom | 1 | <1% |
| Unknown | 131 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 47 | 35% |
| Researcher | 19 | 14% |
| Student > Master | 17 | 13% |
| Student > Bachelor | 11 | 8% |
| Student > Postgraduate | 6 | 4% |
| Other | 15 | 11% |
| Unknown | 21 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 69 | 51% |
| Biochemistry, Genetics and Molecular Biology | 21 | 15% |
| Agricultural and Biological Sciences | 16 | 12% |
| Immunology and Microbiology | 1 | <1% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | <1% |
| Other | 2 | 1% |
| Unknown | 26 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2024.
All research outputs
#7,960,512
of 25,374,647 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#1,095
of 3,834 outputs
Outputs of similar age
#67,516
of 252,061 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#9
of 35 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,834 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,061 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.