Title |
Purine metabolism and the microaerophily of Helicobacter pylori
|
---|---|
Published in |
Archives of Microbiology, December 1997
|
DOI | 10.1007/s002030050521 |
Pubmed ID | |
Authors |
G. L. Mendz, Andrew J. Shepley, Stuart L. Hazell, Mark A. Smith |
Abstract |
The requirements for purine nucleotide synthesis, the effects of purine analogues, and the metabolism of adenine in the bacterium Helicobacter pylori were investigated employing cell culture techniques and one-dimensional NMR spectroscopy. Bacterial cells grew and proliferated in media lacking preformed purines, indicating that H. pylori can synthesize purine nucleotides de novo to meet its requirements. Blocking of this pathway in the absence of sufficient preformed purines for salvage nucleotide synthesis led to cell death. Analogues of purine nucleobases and nucleosides taken up by the cells were cytotoxic, suggesting that salvage routes could be exploited for therapy. Adenine or hypoxanthine were able to substitute for catalase in supporting cell growth and proliferation, suggesting a role for these bases in maintaining the microaerophilic conditions essentially required by the bacterium. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 28% |
Researcher | 7 | 28% |
Student > Bachelor | 3 | 12% |
Student > Master | 2 | 8% |
Professor | 2 | 8% |
Other | 0 | 0% |
Unknown | 4 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 28% |
Biochemistry, Genetics and Molecular Biology | 4 | 16% |
Immunology and Microbiology | 3 | 12% |
Medicine and Dentistry | 3 | 12% |
Chemistry | 2 | 8% |
Other | 0 | 0% |
Unknown | 6 | 24% |