| Title |
Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation
|
|---|---|
| Published in |
Journal of Applied Genetics, November 2010
|
| DOI | 10.1007/s13353-010-0001-5 |
| Pubmed ID | |
| Authors |
Tomasz Pniewski, Józef Kapusta, Piotr Bociąg, Jacek Wojciechowicz, Anna Kostrzak, Michał Gdula, Olga Fedorowicz-Strońska, Piotr Wójcik, Halina Otta, Sławomir Samardakiewicz, Bogdan Wolko, Andrzej Płucienniczak |
| Abstract |
Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Egypt | 1 | 1% |
| Unknown | 71 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 14 | 19% |
| Researcher | 12 | 17% |
| Student > Bachelor | 12 | 17% |
| Student > Master | 10 | 14% |
| Student > Doctoral Student | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 17 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 40% |
| Biochemistry, Genetics and Molecular Biology | 13 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
| Veterinary Science and Veterinary Medicine | 2 | 3% |
| Engineering | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 18 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2023.
All research outputs
#4,324,162
of 23,445,423 outputs
Outputs from Journal of Applied Genetics
#32
of 400 outputs
Outputs of similar age
#27,661
of 182,879 outputs
Outputs of similar age from Journal of Applied Genetics
#1
of 6 outputs
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So far Altmetric has tracked 400 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done well, scoring higher than 89% of its peers.
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