| Title |
Coordinated regulation of autophagy and apoptosis determines endothelial cell fate during Dengue virus type 2 infection
|
|---|---|
| Published in |
Molecular and Cellular Biochemistry, August 2014
|
| DOI | 10.1007/s11010-014-2183-3 |
| Pubmed ID | |
| Authors |
Junqi Huang, Ying Li, Yiming Qi, Yingke Zhang, Lin Zhang, Zilian Wang, Xuzhi Zhang, Lian Gui |
| Abstract |
Dengue is the most prevalent mosquito-borne viral disease in tropical regions. Severe cases may progress to Dengue hemorrhagic fever, suggesting vascular endothelial dysfunction in disease pathogenesis. In our previous study, we found that Dengue virus type 2 (DENV2) induced apoptosis of vascular endothelial cells via FasL/Fas- and XIAP-associated factor 1 (XAF1)-dependent pathways. In this paper, we demonstrate that DENV2 can induce autophagy in primary human umbilical vein endothelial cells (HUVECs) and the human umbilical vein endothelial cell line EA.hy926. Inhibition of autophagy with 3-methyl adenine promoted apoptosis, while inhibition of apoptosis with Z-VAD-FMK facilitated autophagy in DENV2-infected HUVECs and EA.hy926 cells. Interferon-alpha-inducible protein 6 (IFI6), a putative apoptosis regulator, inhibited DENV2-induced autophagy in EA.hy926 cells, while XAF1, an inhibitor of anti-apoptotic XIAP, facilitated autophagy. Molecular regulators of apoptosis and autophagy interact at multiple levels to determine cell fate. Our data suggest that XAF1 and IFI6 are involved in regulating the balance between autophagy and apoptosis in DENV2-infected endothelial cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 19% |
| Student > Ph. D. Student | 8 | 15% |
| Researcher | 7 | 13% |
| Student > Bachelor | 6 | 11% |
| Professor > Associate Professor | 4 | 7% |
| Other | 8 | 15% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 28% |
| Biochemistry, Genetics and Molecular Biology | 9 | 17% |
| Medicine and Dentistry | 9 | 17% |
| Immunology and Microbiology | 4 | 7% |
| Nursing and Health Professions | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 12 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2016.
All research outputs
#2,916,997
of 22,851,489 outputs
Outputs from Molecular and Cellular Biochemistry
#88
of 2,307 outputs
Outputs of similar age
#31,217
of 235,803 outputs
Outputs of similar age from Molecular and Cellular Biochemistry
#3
of 32 outputs
Altmetric has tracked 22,851,489 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,307 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 235,803 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.