Title |
NBR1 enables autophagy-dependent focal adhesion turnover
|
---|---|
Published in |
Journal of Cell Biology, February 2016
|
DOI | 10.1083/jcb.201503075 |
Pubmed ID | |
Authors |
Candia M. Kenific, Samantha J. Stehbens, Juliet Goldsmith, Andrew M. Leidal, Nathalie Faure, Jordan Ye, Torsten Wittmann, Jayanta Debnath |
Abstract |
Autophagy is a catabolic pathway involving the sequestration of cellular contents into a double-membrane vesicle, the autophagosome. Although recent studies have demonstrated that autophagy supports cell migration, the underlying mechanisms remain unknown. Using live-cell imaging, we uncover that autophagy promotes optimal migratory rate and facilitates the dynamic assembly and disassembly of cell-matrix focal adhesions (FAs), which is essential for efficient motility. Additionally, our studies reveal that autophagosomes associate with FAs primarily during disassembly, suggesting autophagy locally facilitates the destabilization of cell-matrix contact sites. Furthermore, we identify the selective autophagy cargo receptor neighbor of BRCA1 (NBR1) as a key mediator of autophagy-dependent FA remodeling. NBR1 depletion impairs FA turnover and decreases targeting of autophagosomes to FAs, whereas ectopic expression of autophagy-competent, but not autophagy-defective, NBR1 enhances FA disassembly and reduces FA lifetime during migration. Our findings provide mechanistic insight into how autophagy promotes migration by revealing a requirement for NBR1-mediated selective autophagy in enabling FA disassembly in motile cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 29% |
Finland | 1 | 14% |
Australia | 1 | 14% |
United Kingdom | 1 | 14% |
Sweden | 1 | 14% |
Unknown | 1 | 14% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 57% |
Scientists | 2 | 29% |
Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
United States | 1 | <1% |
Unknown | 170 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 34 | 20% |
Student > Ph. D. Student | 32 | 19% |
Researcher | 22 | 13% |
Student > Bachelor | 19 | 11% |
Student > Doctoral Student | 11 | 6% |
Other | 18 | 10% |
Unknown | 36 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 52 | 30% |
Agricultural and Biological Sciences | 45 | 26% |
Medicine and Dentistry | 9 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 5% |
Neuroscience | 4 | 2% |
Other | 14 | 8% |
Unknown | 40 | 23% |