Title |
Double conjugation strategy to incorporate lipid adjuvants into multiantigenic vaccines
|
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Published in |
Chemical Science, January 2016
|
DOI | 10.1039/c5sc03859f |
Pubmed ID | |
Authors |
Waleed M. Hussein, Tzu-Yu Liu, Pirashanthini Maruthayanar, Saori Mukaida, Peter M. Moyle, James W. Wells, Istvan Toth, Mariusz Skwarczynski |
Abstract |
Conjugation of multiple peptides by their N-termini is a promising technique to produce branched multiantigenic vaccines. We established a double conjugation strategy that combines a mercapto-acryloyl Michael addition and a copper-catalysed alkyne-azide 1,3-dipolar cycloaddition (CuAAC) reaction to synthesise self-adjuvanting branched multiantigenic vaccine candidates. These vaccine candidates aim to treat cervical cancer and include two HPV-16 derived epitopes and a novel self-adjuvanting moiety. This is the first report of mercapto-acryloyl conjugation applied to the hetero conjugation of two unprotected peptides by their N-termini followed by a CuAAC reaction to conjugate a novel synthetic lipoalkyne self-adjuvanting moiety. In vivo experiments showed that the most promising vaccine candidate completely eradicated tumours in 46% of the mice (6 out of 13 mice). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 38% |
Researcher | 2 | 13% |
Other | 1 | 6% |
Student > Master | 1 | 6% |
Professor | 1 | 6% |
Other | 2 | 13% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 10 | 63% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Chemical Engineering | 1 | 6% |
Unknown | 3 | 19% |