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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP
|
|---|---|
| Published in |
The Journal of Physical Chemistry Letters, February 2016
|
| DOI | 10.1021/acs.jpclett.5b02789 |
| Pubmed ID | |
| Authors |
Jacques-Philippe Colletier, Michel Sliwa, François-Xavier Gallat, Michihiro Sugahara, Virginia Guillon, Giorgio Schirò, Nicolas Coquelle, Joyce Woodhouse, Laure Roux, Guillaume Gotthard, Antoine Royant, Lucas Martinez Uriarte, Cyril Ruckebusch, Yasumasa Joti, Martin Byrdin, Eiichi Mizohata, Eriko Nango, Tomoyuki Tanaka, Kensuke Tono, Makina Yabashi, Virgile Adam, Marco Cammarata, Ilme Schlichting, Dominique Bourgeois, Martin Weik |
| Abstract |
Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultra-fast photochemical time scale, remain unknown. We employed time-resolved pump-probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the non-fluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond time scales that are attributed to chromophore isomerization and proton transfer, respectively. Kinetic modeling favors a sequential mechanism with the existence of two excited state in-termediates with lifetimes of 2 and 15 ps, the second of which controls the photoswitching quantum yield. In order to support that IrisFP is suited for time-resolved experiments aiming at a structural characterization of these ps intermediates, we used serial femtosecond crys-tallography at an X-ray free electron laser and solved the structure of IrisFP in its on state. Sample consumption was minimized by embedding crystals in mineral grease - in which they remain photoswitchable. Our spectro-scopic and structural results pave the way for time-resolved serial femtosecond crystallography aiming at characterizing the structure of ultrafast intermediates in reversibly photoswitchable fluorescent proteins. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 88 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 25 | 28% |
| Student > Ph. D. Student | 20 | 22% |
| Student > Master | 7 | 8% |
| Student > Bachelor | 5 | 6% |
| Professor | 5 | 6% |
| Other | 9 | 10% |
| Unknown | 19 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 31 | 34% |
| Biochemistry, Genetics and Molecular Biology | 15 | 17% |
| Agricultural and Biological Sciences | 13 | 14% |
| Physics and Astronomy | 8 | 9% |
| Engineering | 2 | 2% |
| Other | 3 | 3% |
| Unknown | 18 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2016.
All research outputs
#14,388,865
of 25,377,790 outputs
Outputs from The Journal of Physical Chemistry Letters
#3,282
of 10,169 outputs
Outputs of similar age
#143,026
of 312,897 outputs
Outputs of similar age from The Journal of Physical Chemistry Letters
#39
of 127 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,169 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,897 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 127 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.