| Title |
Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype
|
|---|---|
| Published in |
BMC Infectious Diseases, February 2016
|
| DOI | 10.1186/s12879-016-1431-4 |
| Pubmed ID | |
| Authors |
Lourimar Viana Nascimento F. de Sousa, Vera Lúcia Santos, Andrea de Souza Monteiro, Marcus Vinicíus Dias-Souza, Sirlei Garcia Marques, Elaine Speziali de Faria, Elaine Alves de Oliveira Assunção, Simone Gonçalves dos Santos, Juan Moises Zonis, Daniel Gomes de Alvarenga, Rodrigo Assunção de Holanda, Jaqueline Gontijo de Sousa, Kênia Valéria dos Santos, Maria Aparecida de Resende Stoianoff |
| Abstract |
Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients. The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay. C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17. The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 60 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 18% |
| Student > Ph. D. Student | 8 | 13% |
| Researcher | 7 | 12% |
| Student > Bachelor | 7 | 12% |
| Professor | 4 | 7% |
| Other | 10 | 17% |
| Unknown | 13 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 13 | 22% |
| Immunology and Microbiology | 12 | 20% |
| Biochemistry, Genetics and Molecular Biology | 8 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
| Nursing and Health Professions | 2 | 3% |
| Other | 4 | 7% |
| Unknown | 18 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2016.
All research outputs
#18,444,553
of 22,852,911 outputs
Outputs from BMC Infectious Diseases
#5,609
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Outputs of similar age
#216,913
of 298,745 outputs
Outputs of similar age from BMC Infectious Diseases
#68
of 91 outputs
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