| Title |
A novel curcumin-like dienone induces apoptosis in triple-negative breast cancer cells
|
|---|---|
| Published in |
Cellular Oncology, February 2016
|
| DOI | 10.1007/s13402-016-0272-x |
| Pubmed ID | |
| Authors |
Elisa Robles-Escajeda, Umashankar Das, Nora M. Ortega, Karla Parra, Giulio Francia, Jonathan R. Dimmock, Armando Varela-Ramirez, Renato J. Aguilera |
| Abstract |
According to the World Health Organization (WHO), breast cancer is the most common cancer affecting women worldwide. In the USA ~12.3 % of all women are expected to be diagnosed with various types of breast cancer, exhibiting varying degrees of therapeutic response rates. Therefore, the identification of novel anti-breast cancer drugs is of paramount importance. The 1,5-diaryl-3-oxo-1,4-pentadienyl pharmacophore was incorporated into a number of cytotoxins. Three of the resulting dienones, 2a, 2b and 2c, were tested for their anti-neoplastic potencies in a variety of human breast cancer-derived cell lines, including the triple negative MDA-MB-231 cell line and its metastatic variant, using a live-cell bio-imaging method. Special emphasis was put on dienone 2c, since its anti-cancer activity and its mode of inflicting cell death have so far not been reported. We found that all three dienones exhibited potent cytotoxicities towards the breast cancer-derived cell lines tested, whereas significantly lower toxicities were observed towards the non-cancerous human breast cell line MCF-10A. The dienones 2b and 2c exhibited the greatest selective cytotoxicity at submicromolar concentration levels. We found that these two dienones induced phosphatidylserine externalization in MDA-MB-231 cells in a concentration-dependent manner, suggesting that their cytotoxic effect might be mediated by apoptosis. This possibility was confirmed by our observation that the dienone 2c can induce mitochondrial depolarization, caspase-3 activation, cell cycle disruption and DNA fragmentation in MDA-MB-231 cells. Our findings indicate that dienone 2c uses the mitochondrial/intrinsic pathway to inflict apoptosis in triple negative MDA-MB-231 breast cancer-derived cells. This observation warrants further assessment of dienone 2c as a potential anti-breast cancer drug. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 20% |
| Student > Master | 9 | 20% |
| Student > Bachelor | 6 | 14% |
| Student > Doctoral Student | 3 | 7% |
| Researcher | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 11 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 14% |
| Chemistry | 4 | 9% |
| Medicine and Dentistry | 2 | 5% |
| Nursing and Health Professions | 1 | 2% |
| Other | 6 | 14% |
| Unknown | 15 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2016.
All research outputs
#19,516,978
of 23,999,200 outputs
Outputs from Cellular Oncology
#259
of 426 outputs
Outputs of similar age
#221,593
of 301,569 outputs
Outputs of similar age from Cellular Oncology
#5
of 10 outputs
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