Title |
miR-139-5p suppresses cancer cell migration and invasion through targeting ZEB1 and ZEB2 in GBM
|
---|---|
Published in |
Tumor Biology, April 2015
|
DOI | 10.1007/s13277-015-3372-8 |
Pubmed ID | |
Authors |
Sihai Yue, Lihua Wang, Hui Zhang, Youhui Min, Yongli Lou, Hongshan Sun, Yu Jiang, Wenjin Zhang, Aming Liang, Yongkun Guo, Ping Chen, Guowei Lv, Liuxiang Wang, Qinghua Zong, Yong Li |
Abstract |
Invasion and migration of glioblastoma multiforme (GBM) is a multistep process and an important phenotype that causes this disease to invade surrounding tissues in the brain. Recent studies have highlighted that miRNAs play a pivotal role in controlling GBM cell plasticity. In this report, we used wound healing and transwell assays to identify a novel role of miR-139-5p in inhibition of GBM cell migration and invasion. Bioinformatics coupled with luciferase and Western blot assays also revealed that miR-139-5p inhibited expression of ZEB1 and ZEB2, which are master regulators of tumor metastasis. MiR-139-5p specifically interacts with the 3'-UTR regions of ZEB1 and ZEB2, attenuating their expression in GBM cells. To corroborate this finding, we rescued ZEB1 and ZEB2 expression and found partial but significant increases in miR-139-5p-suppressed invasion of GBM cells. The biological relevance of our study was validated by analyzing levels of miR-139-5p in GBM tissue. We found that its expression significantly downregulated compared to normal tissue and shorter overall survival rates in patients with lower miR-139-5p expression. These results confirm that miR-139-5p suppresses GBM migration and invasion and highlight its potential as a biomarker and therapeutic target for treating GBM. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 33% |
Portugal | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 24% |
Student > Ph. D. Student | 4 | 19% |
Student > Bachelor | 3 | 14% |
Other | 3 | 14% |
Student > Postgraduate | 1 | 5% |
Other | 0 | 0% |
Unknown | 5 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 29% |
Medicine and Dentistry | 5 | 24% |
Neuroscience | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 8 | 38% |