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Co-occurrence of analogous enzymes determines evolution of a novel (βα)8-isomerase sub-family after non-conserved mutations in flexible loop.

Overview of attention for article published in Biochemical Journal, April 2016
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Title
Co-occurrence of analogous enzymes determines evolution of a novel (βα)8-isomerase sub-family after non-conserved mutations in flexible loop.
Published in
Biochemical Journal, April 2016
DOI 10.1042/bj20151271
Pubmed ID
Authors

Ernesto A Verduzco-Castro, Karolina Michalska, Michael Endres, Ana L Juárez-Vazquez, Lianet Noda-García, Changsoo Chang, Christopher S Henry, Gyorgy Babnigg, Andrzej Joachimiak, Francisco Barona-Gómez

Abstract

We investigate the evolution of co-occurring analogous enzymes involved in L-tryptophan and L-histidine biosynthesis in Actinobacteria . Phylogenetic analysis of trpF homologs, a missing gene in certain clades of this lineage whose absence is complemented by a dual-substrate HisA homolog, termed PriA, found that they fall into three categories: (i) trpF-1 , an L-tryptophan biosynthetic gene horizontally acquired by certain Corynebacterium species; (ii) trpF-2 , a paralog known to be involved in synthesizing a pyrrolopyrrole moiety; and (iii) trpF-3 , a variable non-conserved ortholog of trpF-1 . We previously investigated the effect of trpF-1 upon the evolution of PriA substrate specificity, but nothing is known about the relationship between trpF-3 and priA . After in vitro steady-state enzyme kinetics we found that trpF-3 encodes for a phosphoribosyl anthranilate isomerase. However, mutation of this gene in Streptomyces sviceus did not lead to auxothrophy, as expected from the biosynthetic role of trpF-1 . Biochemical characterization of a dozen co-occurring TrpF-2 or TrpF-3, with PriA homologs, explained the prototrophic phenotype, and unveiled an enzyme activity trade-off between TrpF and PriA. X-ray structural analysis suggests that the function of these PriA homologs is mediated by non-conserved mutations in the flexible L5 loop, which may be responsible for different substrate affinities. Thus, the PriA homologs that co-occur with TrpF-3 represent a novel enzyme family, termed PriB, which evolved in response to PRA isomerase activity. The characterization of co-occurring enzymes provides insights into the influence of functional redundancy on the evolution of enzyme function, which could be useful for enzyme functional annotation.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 33%
Researcher 5 24%
Student > Doctoral Student 3 14%
Student > Master 1 5%
Student > Bachelor 1 5%
Other 2 10%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 43%
Agricultural and Biological Sciences 8 38%
Chemistry 1 5%
Engineering 1 5%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2016.
All research outputs
#15,362,070
of 22,852,911 outputs
Outputs from Biochemical Journal
#10,017
of 11,408 outputs
Outputs of similar age
#179,350
of 298,881 outputs
Outputs of similar age from Biochemical Journal
#51
of 74 outputs
Altmetric has tracked 22,852,911 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,408 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,881 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.