| Title |
Molecular Studies on the Transmission of Indian Cassava Mosaic Virus (ICMV) and Sri Lankan Cassava Mosaic Virus (SLCMV) in Cassava by Bemisia tabaci and Cloning of ICMV and SLCMV Replicase Gene from Cassava
|
|---|---|
| Published in |
Molecular Biotechnology, February 2012
|
| DOI | 10.1007/s12033-012-9503-1 |
| Pubmed ID | |
| Authors |
Raghu Duraisamy, Senthil Natesan, Raveendran Muthurajan, Karthikayan Gandhi, Pugalendhi Lakshmanan, Nageswari Karuppusamy, Mohan Chokkappan |
| Abstract |
Cassava mosaic disease, caused by cassava mosaic geminiviruses are transmitted by Bemisia tabaci. The B. tabaci adults from colonies reared on virus free cassava plant produced from apical meristem culture was studied to determine their ability to transmit Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV) from cassava to cassava. Virus free plants were confirmed by polymerase chain reaction (PCR) using geminivirus degenerate primers. The virus acquisition access period (AAP) of 48 h on virus infected cassava leaves and 48 h virus inoculation access periods on virus free healthy leaves were investigated. Both ICMV and SLCMV were absolutely transmitted by whiteflies reared on cassava. Virus specific primers were designed in the replicase region and used to detect virus in B. tabaci after different AAP. The PCR amplified replicase genes from virus transmitted cassava leaves were cloned the plasmid DNA was isolated from a recombinant colony of E. coli DH5α after their confirmation by colony PCR and sequenced them. The nucleotide sequences obtained from automated DNA sequencing were confirmed as ICMV and SLCMV replicase gene after homology searching by BLAST and found to be a new isolates. The nucleotide sequences of new isolates were submitted in GenBank (accession number JN652126 and JN595785). |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 56 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 18% |
| Student > Master | 9 | 16% |
| Student > Ph. D. Student | 7 | 13% |
| Professor | 5 | 9% |
| Student > Doctoral Student | 3 | 5% |
| Other | 6 | 11% |
| Unknown | 16 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 50% |
| Biochemistry, Genetics and Molecular Biology | 4 | 7% |
| Medicine and Dentistry | 2 | 4% |
| Chemical Engineering | 1 | 2% |
| Earth and Planetary Sciences | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 19 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2012.
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#15,242,272
of 22,663,150 outputs
Outputs from Molecular Biotechnology
#643
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Outputs of similar age
#163,769
of 247,566 outputs
Outputs of similar age from Molecular Biotechnology
#12
of 13 outputs
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