| Title |
Particle size measurement of lipoprotein fractions using diffusion-ordered NMR spectroscopy
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2012
|
| DOI | 10.1007/s00216-011-5705-9 |
| Pubmed ID | |
| Authors |
Roger Mallol, Miguel A. Rodríguez, Mercedes Heras, Maria Vinaixa, Núria Plana, Lluís Masana, Gareth A. Morris, Xavier Correig |
| Abstract |
The sizes of certain types of lipoprotein particles have been associated with an increased risk of cardiovascular disease. However, there is currently no gold standard technique for the determination of this parameter. Here, we propose an analytical procedure to measure lipoprotein particles sizes using diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY). The method was tested on six lipoprotein fractions, VLDL, IDL, LDL1, LDL2, HDL2, and HDL3, which were obtained by sequential ultracentrifugation from four patients. We performed a pulsed-field gradient experiment on each fraction to obtain a mean diffusion coefficient, and then determined the apparent hydrodynamic radius using the Stokes–Einstein equation. To validate the hydrodynamic radii obtained, the particle size distribution of these lipoprotein fractions was also measured using transmission electron microscopy (TEM). The standard errors of duplicate measurements of diffusion coefficient ranged from 0.5% to 1.3%, confirming the repeatability of the technique. The coefficient of determination between the hydrodynamic radii and the TEM-derived mean particle size was r2 = 0.96, and the agreement between the two techniques was 85%. Thus, DOSY experiments have proved to be accurate and reliable for estimating lipoprotein particle sizes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 4% |
| Spain | 1 | 1% |
| Estonia | 1 | 1% |
| France | 1 | 1% |
| Unknown | 67 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 22% |
| Student > Ph. D. Student | 15 | 21% |
| Student > Master | 6 | 8% |
| Student > Doctoral Student | 6 | 8% |
| Professor | 5 | 7% |
| Other | 19 | 26% |
| Unknown | 6 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 15 | 21% |
| Agricultural and Biological Sciences | 12 | 16% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Engineering | 8 | 11% |
| Medicine and Dentistry | 6 | 8% |
| Other | 8 | 11% |
| Unknown | 14 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2021.
All research outputs
#4,835,157
of 25,371,288 outputs
Outputs from Analytical & Bioanalytical Chemistry
#727
of 9,618 outputs
Outputs of similar age
#38,793
of 253,515 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#8
of 85 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,618 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 92% of its peers.
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We're also able to compare this research output to 85 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.