Title |
Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform
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Published in |
Lab on a Chip - Miniaturisation for Chemistry & Biology, February 2012
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DOI | 10.1039/c2lc21170j |
Pubmed ID | |
Authors |
Robert Burger, Patrick Reith, Gregor Kijanka, Victor Akujobi, Patrick Abgrall, Jens Ducrée |
Abstract |
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 2% |
Ireland | 1 | 1% |
Unknown | 88 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 33 | 36% |
Student > Master | 18 | 20% |
Researcher | 14 | 15% |
Student > Bachelor | 8 | 9% |
Professor | 3 | 3% |
Other | 7 | 8% |
Unknown | 8 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 44 | 48% |
Agricultural and Biological Sciences | 15 | 16% |
Physics and Astronomy | 8 | 9% |
Biochemistry, Genetics and Molecular Biology | 5 | 5% |
Chemistry | 4 | 4% |
Other | 8 | 9% |
Unknown | 7 | 8% |