| Title |
Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man
|
|---|---|
| Published in |
Cell and Tissue Research, March 2016
|
| DOI | 10.1007/s00441-016-2359-0 |
| Pubmed ID | |
| Authors |
Wei Liu, Fredrik Edin, Hans Blom, Peetra Magnusson, Annelies Schrott-Fischer, Rudolf Glueckert, Peter A. Santi, Hao Li, Göran Laurell, Helge Rask-Andersen |
| Abstract |
Globally 360 million people have disabling hearing loss and, of these, 32 million are children. Human hearing relies on 15,000 hair cells that transduce mechanical vibrations to electrical signals in the auditory nerve. The process is powered by the endo-cochlear potential, which is produced by a vascularized epithelium that actively transports ions in conjunction with a gap junction (GJ) system. This "battery" is located "off-site" in the lateral wall of the cochlea. The GJ syncytium contains the GJ protein genes beta 2 (GJB2/connexin26 (Cx26)) and 6 (GJB6/connexin30 (Cx30)), which are commonly involved in hereditary deafness. Because the molecular arrangement of these proteins is obscure, we analyze GJ protein expression (Cx26/30) in human cochleae by using super-resolution structured illumination microscopy. At this resolution, the Cx26 and Cx30 proteins were visible as separate plaques, rather than being co-localized in heterotypic channels, as previously suggested. The Cx26 and Cx30 proteins thus seem not to be co-expressed but to form closely associated assemblies of GJ plaques. These results could assist in the development of strategies to treat genetic hearing loss in the future. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Germany | 1 | 3% |
| Unknown | 38 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 28% |
| Other | 4 | 10% |
| Student > Ph. D. Student | 4 | 10% |
| Unspecified | 3 | 8% |
| Lecturer | 1 | 3% |
| Other | 4 | 10% |
| Unknown | 13 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 15% |
| Medicine and Dentistry | 5 | 13% |
| Biochemistry, Genetics and Molecular Biology | 4 | 10% |
| Neuroscience | 4 | 10% |
| Unspecified | 3 | 8% |
| Other | 3 | 8% |
| Unknown | 15 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2017.
All research outputs
#19,236,357
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,706
of 2,279 outputs
Outputs of similar age
#220,063
of 301,221 outputs
Outputs of similar age from Cell and Tissue Research
#15
of 27 outputs
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