| Title |
Screening Anti-Cancer Drugs against Tubulin using Catch-and-Release Electrospray Ionization Mass Spectrometry
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, March 2016
|
| DOI | 10.1007/s13361-016-1360-x |
| Pubmed ID | |
| Authors |
Reza Rezaei Darestani, Philip Winter, Elena N. Kitova, Jack A. Tuszynski, John S. Klassen |
| Abstract |
Tubulin, which is the building block of microtubules, plays an important role in cell division. This critical role makes tubulin an attractive target for the development of chemotherapeutic drugs to treat cancer. Currently, there is no general binding assay for tubulin-drug interactions. The present work describes the application of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay to investigate the binding of colchicinoid drugs to αβ-tubulin dimers extracted from porcine brain. Proof-of-concept experiments using positive (ligands with known affinities) and negative (non-binders) controls were performed to establish the reliability of the assay. The assay was then used to screen a library of seven colchicinoid analogues to test their binding to tubulin and to rank their affinities. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | 67% |
| Portugal | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 5 | 24% |
| Student > Ph. D. Student | 4 | 19% |
| Researcher | 2 | 10% |
| Other | 1 | 5% |
| Unspecified | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 6 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 24% |
| Engineering | 3 | 14% |
| Medicine and Dentistry | 3 | 14% |
| Agricultural and Biological Sciences | 2 | 10% |
| Unspecified | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2020.
All research outputs
#6,442,648
of 25,368,786 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#850
of 3,832 outputs
Outputs of similar age
#83,902
of 313,443 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#5
of 36 outputs
Altmetric has tracked 25,368,786 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 3,832 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,443 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.