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Preface.

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Cover of 'Preface.'

Table of Contents

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    Book Overview
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    Chapter 1 RNA-Protein Complexes and Interactions
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    Chapter 2 Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
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    Chapter 3 Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro
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    Chapter 4 Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
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    Chapter 5 Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
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    Chapter 6 Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
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    Chapter 7 RNA-Protein Complexes and Interactions
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    Chapter 8 Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
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    Chapter 9 RNA-Protein Complexes and Interactions
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    Chapter 10 Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture
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    Chapter 11 RNA-Protein Complexes and Interactions
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    Chapter 12 RNA-Protein Complexes and Interactions
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    Chapter 13 RNA-Protein Complexes and Interactions
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    Chapter 14 Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks
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    Chapter 15 RNA-Protein Complexes and Interactions
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    Chapter 16 Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
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    Chapter 17 RNA-Protein Complexes and Interactions
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    Chapter 18 RNA-Protein Complexes and Interactions
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    Chapter 19 Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5′ Splice-Site Base-Pairing
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    Chapter 20 Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter
Attention for Chapter 17: RNA-Protein Complexes and Interactions
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Chapter title
RNA-Protein Complexes and Interactions
Chapter number 17
Book title
RNA-Protein Complexes and Interactions
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3591-8_17
Pubmed ID
Book ISBNs
978-1-4939-3589-5, 978-1-4939-3591-8
Authors

Weyn-Vanhentenryck, Sebastien M, Zhang, Chaolin, Sebastien M. Weyn-Vanhentenryck, Chaolin Zhang, Weyn-Vanhentenryck, Sebastien M.

Abstract

RNA-binding proteins (RBPs) are critical components of post-transcriptional gene expression regulation. However, their binding sites have until recently been difficult to determine due to the apparent low specificity of RBPs for their target transcripts and the lack of high-throughput assays for analyzing binding sites genome wide. Here we present a bioinformatics method for predicting RBP binding motif sites on a genome-wide scale that leverages motif conservation, RNA secondary structure, and the tendency of RBP binding sites to cluster together. A probabilistic model is learned from bona fide binding sites determined by CLIP and applied genome wide to generate high specificity binding site predictions.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Researcher 3 27%
Professor 2 18%
Student > Doctoral Student 1 9%
Professor > Associate Professor 1 9%
Other 0 0%
Unknown 1 9%
Readers by discipline Count As %
Computer Science 2 18%
Neuroscience 2 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Earth and Planetary Sciences 1 9%
Biochemistry, Genetics and Molecular Biology 1 9%
Other 2 18%
Unknown 2 18%
Attention Score in Context

Attention Score in Context

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#18,447,592
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Outputs of similar age from Methods in molecular biology
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