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Attention Score in Context
| Title |
Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay
|
|---|---|
| Published in |
Analytical Biochemistry, March 2016
|
| DOI | 10.1016/j.ab.2016.02.020 |
| Pubmed ID | |
| Authors |
Yanyan Zhao, Ying Liang, Yuan Liu, Xiao Zhang, Xiaodan Hu, Sicong Tu, Aihua Wu, Cunzheng Zhang, Jianfeng Zhong, Shengming Zhao, Xianjin Liu, Kang Tu |
| Abstract |
An antibody to phenoxybenzoic acid (PBA), the conserved chemical region of pyrethroids, was developed using a DAB library to enable pyrethroid detection in agricultural products. The domain antibody (DAB) library, constructed without animal immunization and based on a human VH framework, displayed repertoires on filamentous bacteriophage. After four rounds of panning, we obtained five domain antibodies which are capable of binding to PBA. Antibody A3 has strong identification capability to cypermethrin, beta-cypermethrin, and fenvalerate. The antibody A3 was used to develop an enzyme-linked immunosorbent assay (ELISA). The IC50 was 2.586μg/ml, 1.814μg/ml and 2.251μg/ml for cypermethrin, beta-cypermethrin and fenvalerate, respectively. The assay shows weak competition with flucythrinate but no competition with fenpropathrin, deltamethrin and permethrin. The developed ELISA process was successfully applied to fortified Chinese cabbage samples, and the recoveries of cypermethrin, beta-cypermethrin, and fenvalerate ranging from 84.4% to 112.3%. We developed an immunoassay to detect pyrethroids depending on the domain antibody library, which overcomes the limitation of requiring protein antigen to immunize animals raising antibody. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 17% |
| Other | 1 | 8% |
| Librarian | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 3 | 25% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 2 | 17% |
| Veterinary Science and Veterinary Medicine | 1 | 8% |
| Arts and Humanities | 1 | 8% |
| Nursing and Health Professions | 1 | 8% |
| Chemical Engineering | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 4 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2016.
All research outputs
#22,760,732
of 25,374,917 outputs
Outputs from Analytical Biochemistry
#8,127
of 8,534 outputs
Outputs of similar age
#269,698
of 313,323 outputs
Outputs of similar age from Analytical Biochemistry
#25
of 32 outputs
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So far Altmetric has tracked 8,534 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.